Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb

Yuki Hirota, Masato Sawada, Yasuyuki S. Kida, Shih Hui Huang, Osamu Yamada, Masanori Sakaguchi, Toshihiko Ogura, Hideyuki Okano, Kazunobu Sawamoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Neuronal precursor cells generated by stem cells in the subventricular zone (SVZ) migrate and differentiate into mature interneurons in the olfactory bulb (OB). The mechanisms responsible for the dynamic morphological changes in cells during this process are largely unknown. Wnt/planar cell polarity (PCP) signaling regulates various developmental events, including neuronal migration and neurite formation. Here, we studied the function of two components of the PCP pathway, Dishevelled2 and Van Gogh like-2, in the newborn neurons in the postnatal mouse OB. Electroporation- or lentivirus-mediated introduction of vectors carrying a knockdown or dominant-negative construct of these genes into the SVZ altered the distribution and dendrite formation of newborn neurons in the OB, suggesting that PCP signaling is involved in regulating the maturation of new neurons in the OB.

Original languageEnglish
Pages (from-to)1726-1733
Number of pages8
JournalStem Cells
Volume30
Issue number8
DOIs
Publication statusPublished - 2012 Aug

Fingerprint

Cell Polarity
Olfactory Bulb
Lateral Ventricles
Neurons
Lentivirus
Electroporation
Interneurons
Neurites
Dendrites
Stem Cells
Genes

Keywords

  • Dishevelled2
  • Mouse
  • Neurogenesis
  • Olfactory bulb
  • Planar cell polarity pathway
  • Subventricular zone
  • Van Gogh like-2

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Hirota, Y., Sawada, M., Kida, Y. S., Huang, S. H., Yamada, O., Sakaguchi, M., ... Sawamoto, K. (2012). Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb. Stem Cells, 30(8), 1726-1733. https://doi.org/10.1002/stem.1137

Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb. / Hirota, Yuki; Sawada, Masato; Kida, Yasuyuki S.; Huang, Shih Hui; Yamada, Osamu; Sakaguchi, Masanori; Ogura, Toshihiko; Okano, Hideyuki; Sawamoto, Kazunobu.

In: Stem Cells, Vol. 30, No. 8, 08.2012, p. 1726-1733.

Research output: Contribution to journalArticle

Hirota, Y, Sawada, M, Kida, YS, Huang, SH, Yamada, O, Sakaguchi, M, Ogura, T, Okano, H & Sawamoto, K 2012, 'Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb', Stem Cells, vol. 30, no. 8, pp. 1726-1733. https://doi.org/10.1002/stem.1137
Hirota, Yuki ; Sawada, Masato ; Kida, Yasuyuki S. ; Huang, Shih Hui ; Yamada, Osamu ; Sakaguchi, Masanori ; Ogura, Toshihiko ; Okano, Hideyuki ; Sawamoto, Kazunobu. / Roles of planar cell polarity signaling in maturation of neuronal precursor cells in the postnatal mouse olfactory bulb. In: Stem Cells. 2012 ; Vol. 30, No. 8. pp. 1726-1733.
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