Abstract
The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.
| Original language | English |
|---|---|
| Pages (from-to) | 1003-1009 |
| Number of pages | 7 |
| Journal | Placenta |
| Volume | 31 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2010 Nov |
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Keywords
- Betaine
- Hypertonicity
- Syncytiotrophoblast
- System A
- Taurine
- TauT
ASJC Scopus subject areas
- Obstetrics and Gynaecology
- Reproductive Medicine
- Developmental Biology
Cite this
Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress. / Nishimura, Tomohiro; Sai, Y.; Fujii, J.; Muta, M.; Iizasa, H.; Tomi, Masatoshi; Deureh, M.; Kose, N.; Nakashima, E.
In: Placenta, Vol. 31, No. 11, 11.2010, p. 1003-1009.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress
AU - Nishimura, Tomohiro
AU - Sai, Y.
AU - Fujii, J.
AU - Muta, M.
AU - Iizasa, H.
AU - Tomi, Masatoshi
AU - Deureh, M.
AU - Kose, N.
AU - Nakashima, E.
PY - 2010/11
Y1 - 2010/11
N2 - The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.
AB - The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.
KW - Betaine
KW - Hypertonicity
KW - Syncytiotrophoblast
KW - System A
KW - Taurine
KW - TauT
UR - http://www.scopus.com/inward/record.url?scp=78049280148&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049280148&partnerID=8YFLogxK
U2 - 10.1016/j.placenta.2010.08.003
DO - 10.1016/j.placenta.2010.08.003
M3 - Article
VL - 31
SP - 1003
EP - 1009
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 11
ER -