Abstract
The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.
Original language | English |
---|---|
Pages (from-to) | 1003-1009 |
Number of pages | 7 |
Journal | Placenta |
Volume | 31 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2010 Nov 1 |
Keywords
- Betaine
- Hypertonicity
- Syncytiotrophoblast
- System A
- TauT
- Taurine
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology
- Developmental Biology