Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress

T. Nishimura; Y. Sai; J. Fujii; M. Muta; H. Iizasa; M. Tomi; M. Deureh; N. Kose; E. Nakashima

doi:10.1016/j.placenta.2010.08.003

Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress

T. Nishimura, Y. Sai, J. Fujii, M. Muta, H. Iizasa, M. Tomi, M. Deureh, N. Kose, E. Nakashima

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [³H]taurine and [¹⁴C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [³H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [¹⁴C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.

Original language	English
Pages (from-to)	1003-1009
Number of pages	7
Journal	Placenta
Volume	31
Issue number	11
DOIs	https://doi.org/10.1016/j.placenta.2010.08.003
Publication status	Published - 2010 Nov

Keywords

Betaine
Hypertonicity
Syncytiotrophoblast
System A
TauT
Taurine

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynaecology
Developmental Biology

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10.1016/j.placenta.2010.08.003

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title = "Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress",

abstract = "The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.",

keywords = "Betaine, Hypertonicity, Syncytiotrophoblast, System A, TauT, Taurine",

author = "T. Nishimura and Y. Sai and J. Fujii and M. Muta and H. Iizasa and M. Tomi and M. Deureh and N. Kose and E. Nakashima",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan , a grant from the “High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT 2004-2008, the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan , a grant from The Nagai Foundation, Tokyo , and a grant from the Joint Research Project under the Japan-Korea Basic Scientific Cooperation Program of the Japan Society for the Promotion of Science (JSPS) and the Korea Science & Engineering Foundation (KOSEF) .",

year = "2010",

month = nov,

doi = "10.1016/j.placenta.2010.08.003",

language = "English",

volume = "31",

pages = "1003--1009",

journal = "Placenta",

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T1 - Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress

AU - Nishimura, T.

AU - Sai, Y.

AU - Fujii, J.

AU - Muta, M.

AU - Iizasa, H.

AU - Tomi, M.

AU - Deureh, M.

AU - Kose, N.

AU - Nakashima, E.

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan , a grant from the “High-Tech Research Center” Project for Private Universities: matching fund subsidy from MEXT 2004-2008, the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan , a grant from The Nagai Foundation, Tokyo , and a grant from the Joint Research Project under the Japan-Korea Basic Scientific Cooperation Program of the Japan Society for the Promotion of Science (JSPS) and the Korea Science & Engineering Foundation (KOSEF) .

PY - 2010/11

Y1 - 2010/11

N2 - The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.

AB - The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.

KW - Betaine

KW - Hypertonicity

KW - Syncytiotrophoblast

KW - System A

KW - TauT

KW - Taurine

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DO - 10.1016/j.placenta.2010.08.003

M3 - Article

C2 - 20801504

AN - SCOPUS:78049280148

VL - 31

SP - 1003

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JO - Placenta

JF - Placenta

SN - 0143-4004

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ER -

Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress

Abstract

Keywords

ASJC Scopus subject areas

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