Roles of TauT and system A in cytoprotection of rat syncytiotrophoblast cell line exposed to hypertonic stress

T. Nishimura, Y. Sai, J. Fujii, M. Muta, H. Iizasa, M. Tomi, M. Deureh, N. Kose, E. Nakashima

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The purpose of this study was to clarify the cytoprotective mechanism(s) induced in a conditionally immortalized syncytiotrophoblast cell line (TR-TBT 18d-1) exposed to hypertonic conditions. Hypertonicity-induced apoptosis of TR-TBT 18d-1 cells, but this was blocked by addition of 1 mM taurine to the culture medium. TauT-knockdown using siRNA revealed that TauT is a major contributor to taurine uptake by TR-TBT 18d-1 cells, at least under normal conditions. Cellular uptake of [3H]taurine and [14C] betaine by TR-TBT 18d-1 cells cultured under hypertonic conditions was increased compared to that under normal conditions. TauT, BGT-1, ATA2 and HSP70 mRNAs were upregulated by hypertonicity, while OCTN2, ENT1 and CNT1 mRNAs were downregulated. [3H]Taurine uptake was strongly inhibited by TauT inhibitors such as hypotaurine and β-alanine. MeAIB, a system A specific substrate, inhibited hypertonic stress-induced [14C]betaine uptake. These results suggest that TauT and system A play cytoprotective roles in syncytiotrophoblasts exposed to hypertonic stress.

Original languageEnglish
Pages (from-to)1003-1009
Number of pages7
JournalPlacenta
Volume31
Issue number11
DOIs
Publication statusPublished - 2010 Nov 1

Keywords

  • Betaine
  • Hypertonicity
  • Syncytiotrophoblast
  • System A
  • TauT
  • Taurine

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

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