TY - JOUR
T1 - Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions
AU - Takeoka, Shinji
AU - Teramura, Yuji
AU - Okamura, Yosuke
AU - Tsuchida, Eishun
AU - Handa, Makoto
AU - Ikeda, Yasuo
N1 - Funding Information:
The authors thank Dr. M. Murata at Keio University for useful discussions about the functional evaluation of rGPIbα-vesicles and Mitsubishi Pharma for donating rHSA and rGPIbα. This work was supported by Health Science Research Grants (Artificial Platelets), Research on Advanced Medical Technology, Ministry of Health, Labor and Welfare, Japan and grants-in-aid from the Ministry of Education, Culture, Sport, Science and Technology, Japan (No. 11877172). One of the authors (Y.T.) thanks Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2002
Y1 - 2002
N2 - The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.
AB - The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.
KW - Artificial platelets
KW - Fluorescent anisotropy
KW - GPIbα
KW - Glycoprotein
KW - Membrane flexibility
KW - Phospholipid
KW - Platelets
KW - Rolling
KW - Vesicles
KW - Von Willebrand factor
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U2 - 10.1016/S0006-291X(02)00934-8
DO - 10.1016/S0006-291X(02)00934-8
M3 - Article
C2 - 12176049
AN - SCOPUS:0035984323
SN - 0006-291X
VL - 296
SP - 765
EP - 770
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -