Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling

Kent Imaizumi, Koki Fujimori, Seiji Ishii, Asako Otomo, Yasushi Hosoi, Hiroaki Miyajima, Hitoshi Warita, Masashi Aoki, Shinji Hadano, Wado Akamatsu, Hideyuki Okano

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent stem cell (PSC) cultures. Modulation of FGF8 signaling appropriately regulated the R-C markers, and the patterns of global gene expression resembled those of the corresponding areas of human fetal brains. Furthermore, we demonstrated the utility of this culture system in modeling the area-specific forebrain phenotypes [presumptive upper motor neuron (UMN) phenotypes] of amyotrophic lateral sclerosis (ALS). We anticipate that our culture system will contribute to studies of human neurodevelopment and neurological disease modeling.

Original languageEnglish
Article numbere0368-17.2018
JournaleNeuro
Volume5
Issue number2
DOIs
Publication statusPublished - 2018 Mar 1

Keywords

  • Amyotrophic lateral sclerosis
  • Areal patterning
  • Fibroblast growth factor 8
  • Pluripotent stem cell

ASJC Scopus subject areas

  • Neuroscience(all)

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