@article{65bdebaa2b344aaa919e48c601b7f40d,
title = "Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling",
abstract = "The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent stem cell (PSC) cultures. Modulation of FGF8 signaling appropriately regulated the R-C markers, and the patterns of global gene expression resembled those of the corresponding areas of human fetal brains. Furthermore, we demonstrated the utility of this culture system in modeling the area-specific forebrain phenotypes [presumptive upper motor neuron (UMN) phenotypes] of amyotrophic lateral sclerosis (ALS). We anticipate that our culture system will contribute to studies of human neurodevelopment and neurological disease modeling.",
keywords = "Amyotrophic lateral sclerosis, Areal patterning, Fibroblast growth factor 8, Pluripotent stem cell",
author = "Kent Imaizumi and Koki Fujimori and Seiji Ishii and Asako Otomo and Yasushi Hosoi and Hiroaki Miyajima and Hitoshi Warita and Masashi Aoki and Shinji Hadano and Wado Akamatsu and Hideyuki Okano",
note = "Funding Information: Disease Research Utilizing Disease-Specific iPS Cells funded by the Japan Science and Technology Agency (JST)/Japan Agency for Medical Research and Development (AMED) to H.O. and W.A.; by the Practical Research Project for Rare/Intractable Diseases by AMED to H.O and M.A; by the Ice Bucket Challenge Grant from Japan ALS Association to H.O.; and by Keio University Research Grants for Life Science and Medicine to K.I. Funding Information: This work was supported by grants from the New Energy and Industrial Technology Development Organization; the Ministry of Education, Science, Sports and Culture (MEXT) of Japan; and the Ministry of Health, Labour and Welfare (MHLW) of Japan to H.O. and W.A.; by the Program for Intractable Publisher Copyright: {\textcopyright} 2018 Imaizumi et al.",
year = "2018",
month = mar,
day = "1",
doi = "10.1523/ENEURO.0368-17.2018",
language = "English",
volume = "5",
journal = "eNeuro",
issn = "2373-2822",
publisher = "Society for Neuroscience",
number = "2",
}
