Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling

Kent Imaizumi, Koki Fujimori, Seiji Ishii, Asako Otomo, Yasushi Hosoi, Hiroaki Miyajima, Hitoshi Warita, Masashi Aoki, Shinji Hadano, Wado Akamatsu, Hideyuki Okano

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The cerebral cortex is subdivided into distinct areas that have particular functions. The rostrocaudal (R-C) gradient of fibroblast growth factor 8 (FGF8) signaling defines this areal identity during neural development. In this study, we recapitulated cortical R-C patterning in human pluripotent stem cell (PSC) cultures. Modulation of FGF8 signaling appropriately regulated the R-C markers, and the patterns of global gene expression resembled those of the corresponding areas of human fetal brains. Furthermore, we demonstrated the utility of this culture system in modeling the area-specific forebrain phenotypes [presumptive upper motor neuron (UMN) phenotypes] of amyotrophic lateral sclerosis (ALS). We anticipate that our culture system will contribute to studies of human neurodevelopment and neurological disease modeling.

Original languageEnglish
Article numbere0368-17.2018
JournaleNeuro
Volume5
Issue number2
DOIs
Publication statusPublished - 2018 Mar 1

Fingerprint

Fibroblast Growth Factor 8
Pluripotent Stem Cells
Neurons
Phenotype
Amyotrophic Lateral Sclerosis
Motor Neurons
Prosencephalon
Cerebral Cortex
Cell Culture Techniques
Gene Expression
Brain

Keywords

  • Amyotrophic lateral sclerosis
  • Areal patterning
  • Fibroblast growth factor 8
  • Pluripotent stem cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Imaizumi, K., Fujimori, K., Ishii, S., Otomo, A., Hosoi, Y., Miyajima, H., ... Okano, H. (2018). Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling. eNeuro, 5(2), [e0368-17.2018]. https://doi.org/10.1523/ENEURO.0368-17.2018

Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling. / Imaizumi, Kent; Fujimori, Koki; Ishii, Seiji; Otomo, Asako; Hosoi, Yasushi; Miyajima, Hiroaki; Warita, Hitoshi; Aoki, Masashi; Hadano, Shinji; Akamatsu, Wado; Okano, Hideyuki.

In: eNeuro, Vol. 5, No. 2, e0368-17.2018, 01.03.2018.

Research output: Contribution to journalArticle

Imaizumi, K, Fujimori, K, Ishii, S, Otomo, A, Hosoi, Y, Miyajima, H, Warita, H, Aoki, M, Hadano, S, Akamatsu, W & Okano, H 2018, 'Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling', eNeuro, vol. 5, no. 2, e0368-17.2018. https://doi.org/10.1523/ENEURO.0368-17.2018
Imaizumi K, Fujimori K, Ishii S, Otomo A, Hosoi Y, Miyajima H et al. Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling. eNeuro. 2018 Mar 1;5(2). e0368-17.2018. https://doi.org/10.1523/ENEURO.0368-17.2018
Imaizumi, Kent ; Fujimori, Koki ; Ishii, Seiji ; Otomo, Asako ; Hosoi, Yasushi ; Miyajima, Hiroaki ; Warita, Hitoshi ; Aoki, Masashi ; Hadano, Shinji ; Akamatsu, Wado ; Okano, Hideyuki. / Rostrocaudal areal patterning of human PSC-derived cortical neurons by FGF8 signaling. In: eNeuro. 2018 ; Vol. 5, No. 2.
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