TY - JOUR
T1 - Safe and efficient method for cryopreservation of human induced pluripotent stem cell-derived neural stem and progenitor cells by a programmed freezer with a magnetic field
AU - Nishiyama, Yuichiro
AU - Iwanami, Akio
AU - Kohyama, Jun
AU - Itakura, Go
AU - Kawabata, Soya
AU - Sugai, Keiko
AU - Nishimura, Soraya
AU - Kashiwagi, Rei
AU - Yasutake, Kaori
AU - Isoda, Miho
AU - Matsumoto, Morio
AU - Nakamura, Masaya
AU - Okano, Hideyuki
PY - 2015/11/20
Y1 - 2015/11/20
N2 - Stem cells represent a potential cellular resource in the development of regenerative medicine approaches to the treatment of pathologies in which specific cells are degenerated or damaged by genetic abnormality, disease, or injury. Securing sufficient supplies of cells suited to the demands of cell transplantation, however, remains challenging, and the establishment of safe and efficient cell banking procedures is an important goal. Cryopreservation allows the storage of stem cells for prolonged time periods while maintaining them in adequate condition for use in clinical settings. Conventional cryopreservation systems include slow-freezing and vitrification both have advantages and disadvantages in terms of cell viability and/or scalability. In the present study, we developed an advanced slow-freezing technique using a programmed freezer with a magnetic field called Cells Alive System (CAS) and examined its effectiveness on human induced pluripotent stem cell-derived neural stem/progenitor cells (hiPSC-NS/PCs). This system significantly increased cell viability after thawing and had less impact on cellular proliferation and differentiation. We further found that frozen-thawed hiPSC-NS/PCs were comparable with non-frozen ones at the transcriptome level. Given these findings, we suggest that the CAS is useful for hiPSC-NS/PCs banking for clinical uses involving neural disorders and may open new avenues for future regenerative medicine.
AB - Stem cells represent a potential cellular resource in the development of regenerative medicine approaches to the treatment of pathologies in which specific cells are degenerated or damaged by genetic abnormality, disease, or injury. Securing sufficient supplies of cells suited to the demands of cell transplantation, however, remains challenging, and the establishment of safe and efficient cell banking procedures is an important goal. Cryopreservation allows the storage of stem cells for prolonged time periods while maintaining them in adequate condition for use in clinical settings. Conventional cryopreservation systems include slow-freezing and vitrification both have advantages and disadvantages in terms of cell viability and/or scalability. In the present study, we developed an advanced slow-freezing technique using a programmed freezer with a magnetic field called Cells Alive System (CAS) and examined its effectiveness on human induced pluripotent stem cell-derived neural stem/progenitor cells (hiPSC-NS/PCs). This system significantly increased cell viability after thawing and had less impact on cellular proliferation and differentiation. We further found that frozen-thawed hiPSC-NS/PCs were comparable with non-frozen ones at the transcriptome level. Given these findings, we suggest that the CAS is useful for hiPSC-NS/PCs banking for clinical uses involving neural disorders and may open new avenues for future regenerative medicine.
KW - Allogeneic transplantation
KW - Cells Alive System (CAS)
KW - Central nervous system (CNS) disorder
KW - Cryopreservation
KW - Human iPSC-derived neural stem/progenitor cells (hiPSC-NS/PCs)
KW - Magnetic field
KW - Neurosphere
KW - Spinal cord injury (SCI)
UR - http://www.scopus.com/inward/record.url?scp=84960145049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84960145049&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2015.11.011
DO - 10.1016/j.neures.2015.11.011
M3 - Article
C2 - 26804710
AN - SCOPUS:84960145049
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
ER -