Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay

Illia Aota; Chisato Yatsuda; Ei Ichi Izawa

doi:10.1292/jvms.22-0383

Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay

Illia Aota, Chisato Yatsuda, Ei Ichi Izawa

Department of Sociology, Psychology, Education and Human Sciences

Research output: Contribution to journal › Comment/debate › peer-review

1 Citation (Scopus)

Abstract

Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal’s stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported.We aimed tomeasure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10–60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.

Original language	English
Pages (from-to)	71-75
Number of pages	5
Journal	Journal of Veterinary Medical Science
Volume	85
Issue number	1
DOIs	https://doi.org/10.1292/jvms.22-0383
Publication status	Published - 2023

Keywords

bird
corticosterone
enzyme-linked immunoassay
glucocorticoid
stress

ASJC Scopus subject areas

veterinary(all)

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10.1292/jvms.22-0383

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title = "Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay",

abstract = "Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal{\textquoteright}s stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported.We aimed tomeasure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10–60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.",

keywords = "bird, corticosterone, enzyme-linked immunoassay, glucocorticoid, stress",

author = "Illia Aota and Chisato Yatsuda and Izawa, {Ei Ichi}",

note = "Funding Information: ACKNOWLEDGMENTS. This work was financially supported by JSPS KAKENHI (#20H01787, #21H00962, and #21H0442 to EI Funding Information: This work was financially supported by JSPS KAKENHI (#20H01787, #21H00962, and #21H0442 to EI and, # 20J22529 to IA). Publisher Copyright: {\textcopyright} 2023 The Japanese Society of Veterinary Science.",

year = "2023",

doi = "10.1292/jvms.22-0383",

language = "English",

volume = "85",

pages = "71--75",

journal = "Journal of Veterinary Medical Science",

issn = "0916-7250",

publisher = "Japanese Society of Veterinary Science",

number = "1",

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TY - JOUR

T1 - Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay

AU - Aota, Illia

AU - Yatsuda, Chisato

AU - Izawa, Ei Ichi

N1 - Funding Information: ACKNOWLEDGMENTS. This work was financially supported by JSPS KAKENHI (#20H01787, #21H00962, and #21H0442 to EI Funding Information: This work was financially supported by JSPS KAKENHI (#20H01787, #21H00962, and #21H0442 to EI and, # 20J22529 to IA). Publisher Copyright: © 2023 The Japanese Society of Veterinary Science.

PY - 2023

Y1 - 2023

N2 - Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal’s stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported.We aimed tomeasure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10–60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.

AB - Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal’s stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported.We aimed tomeasure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10–60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.

KW - bird

KW - corticosterone

KW - enzyme-linked immunoassay

KW - glucocorticoid

KW - stress

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AN - SCOPUS:85146125627

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JO - Journal of Veterinary Medical Science

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IS - 1

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Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay

Abstract

Keywords

ASJC Scopus subject areas

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