Salivary corticosterone measurement in large-billed crows by enzyme-linked immunosorbent assay

Illia Aota, Chisato Yatsuda, Ei Ichi Izawa

Research output: Contribution to journalComment/debatepeer-review

1 Citation (Scopus)


Salivary corticosteroid measurement, as a surrogate for plasma corticosteroid levels to evaluate an animal’s stress or metabolic state, commonly used in mammals. However, the validity of salivary corticosterone (CORT) measurements in birds has not yet been reported.We aimed tomeasure salivary CORT in crows using a commercially available CORT enzyme-linked immunosorbent assay kit. An adrenocorticotropic hormone (ACTH) challenge experiment using synthetic cosyntropin, an ACTH analogue, was conducted to compare CORT level elevations between the serum and the saliva in a 10–60 min range. Both salivary and blood CORT was significantly elevated 10 min after injecting synthetic cosyntropin. The results supported the validation of salivary CORT as a surrogate for a blood CORT in crows.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalJournal of Veterinary Medical Science
Issue number1
Publication statusPublished - 2023


  • bird
  • corticosterone
  • enzyme-linked immunoassay
  • glucocorticoid
  • stress

ASJC Scopus subject areas

  • veterinary(all)


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