Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line

Makoto Noda, Minoru Ko, Akihiko Ogura, Ding Gan Liu, Takehiko Amano, Toshiya Takano, Yoji Ikawa

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Abstract

The growth-promoting and/or differentiation-blocking activities of Kirsten (Ki-MSV) or Harvey murine sarcoma virus (Ha7-MSV) on various types of cells in vitro are well documented1-8. Here we report an unexpected effect of these viruses on a rat phaeochromocytoma cell line, PC12. PC12 cells, which multiply indefinitely in growth medium, are known to respond to nerve growth factor (NGF) by cessation of cell division and expression of several properties resembling those of differentiated sympathetic neurones9,10. We have found that Ki- and Ha-MSV mimic some, if not all, of the activities of NGF in PC12 cells, and there is evidence that the viral oncogenes, v-Ki-ras and v-Ha-ras, are responsible for this phenomenon. This system may be of value for studying the mechanism of action of the v-ras genes as well as the regulatory mechanism of growth and differentiation in neuronal cells.

Original languageEnglish
Pages (from-to)73-75
Number of pages3
JournalNature
Volume318
Issue number6041
DOIs
Publication statusPublished - 1985

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ras Genes
PC12 Cells
Sarcoma
Nerve Growth Factor
Viruses
Cell Line
Harvey murine sarcoma virus
Growth
Cell Division

ASJC Scopus subject areas

  • General

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Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line. / Noda, Makoto; Ko, Minoru; Ogura, Akihiko; Liu, Ding Gan; Amano, Takehiko; Takano, Toshiya; Ikawa, Yoji.

In: Nature, Vol. 318, No. 6041, 1985, p. 73-75.

Research output: Contribution to journalArticle

Noda, M, Ko, M, Ogura, A, Liu, DG, Amano, T, Takano, T & Ikawa, Y 1985, 'Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line', Nature, vol. 318, no. 6041, pp. 73-75. https://doi.org/10.1038/318073a0
Noda, Makoto ; Ko, Minoru ; Ogura, Akihiko ; Liu, Ding Gan ; Amano, Takehiko ; Takano, Toshiya ; Ikawa, Yoji. / Sarcoma viruses carrying ras oncogenes induce differentiation-associated properties in a neuronal cell line. In: Nature. 1985 ; Vol. 318, No. 6041. pp. 73-75.
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AU - Ikawa, Yoji

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