Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells

Punnee Nusuetrong; Thitima Pengsuparp; Duangdeun Meksuriyen; Masaaki Tanitsu; Haruhisa Kikuchi; Michinao Mizugaki; Ken Ichi Shimazu; Yoshiteru Oshima; Norimichi Nakahata; Makoto Yoshida

doi:10.1248/bpb.31.1115

Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells

Punnee Nusuetrong, Thitima Pengsuparp, Duangdeun Meksuriyen, Masaaki Tanitsu, Haruhisa Kikuchi, Michinao Mizugaki, Ken Ichi Shimazu, Yoshiteru Oshima, Norimichi Nakahata, Makoto Yoshida

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.

Original language	English
Pages (from-to)	1115-1120
Number of pages	6
Journal	Biological and Pharmaceutical Bulletin
Volume	31
Issue number	6
DOIs	https://doi.org/10.1248/bpb.31.1115
Publication status	Published - 2008 Jun
Externally published	Yes

Keywords

Apoptosis
Glutathione
Lipid peroxide
Reactive oxygen species
Satratoxin H

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

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10.1248/bpb.31.1115

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title = "Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells",

abstract = "Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.",

keywords = "Apoptosis, Glutathione, Lipid peroxide, Reactive oxygen species, Satratoxin H",

author = "Punnee Nusuetrong and Thitima Pengsuparp and Duangdeun Meksuriyen and Masaaki Tanitsu and Haruhisa Kikuchi and Michinao Mizugaki and Shimazu, {Ken Ichi} and Yoshiteru Oshima and Norimichi Nakahata and Makoto Yoshida",

year = "2008",

month = jun,

doi = "10.1248/bpb.31.1115",

language = "English",

volume = "31",

pages = "1115--1120",

journal = "Biological and Pharmaceutical Bulletin",

issn = "0918-6158",

publisher = "Pharmaceutical Society of Japan",

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T1 - Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells

AU - Nusuetrong, Punnee

AU - Pengsuparp, Thitima

AU - Meksuriyen, Duangdeun

AU - Tanitsu, Masaaki

AU - Kikuchi, Haruhisa

AU - Mizugaki, Michinao

AU - Shimazu, Ken Ichi

AU - Oshima, Yoshiteru

AU - Nakahata, Norimichi

AU - Yoshida, Makoto

PY - 2008/6

Y1 - 2008/6

N2 - Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.

AB - Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.

KW - Apoptosis

KW - Glutathione

KW - Lipid peroxide

KW - Reactive oxygen species

KW - Satratoxin H

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DO - 10.1248/bpb.31.1115

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AN - SCOPUS:45749109056

SN - 0918-6158

VL - 31

SP - 1115

EP - 1120

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

IS - 6

ER -

Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells

Abstract

Keywords

ASJC Scopus subject areas

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