Scanning electron microscopy of negatively stained catalase on a silicon wafer

Taiji Furuno, Kevin M. Ulmer, Hiroyuki Sasabe

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A highresolution scanning electron microscope capable of 7spatial resolution at 30kV accelerating voltage was used to observe negatively stained protein molecules. Thin platelet crystals, densely packed monolayers, and lowdensity deposits of beef liver catalase were prepared on the surface of silicon wafers and negatively stained with phosphotungstic acid. The tetrameric structure of the catalase molecule was observed for the first time by scanning electron microscopy on the surface of the smooth silicon wafer. Copyright1992 WileyLiss, Inc.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalMicroscopy Research and Technique
Volume21
Issue number1
DOIs
Publication statusPublished - 1992 Mar 1

Keywords

  • Highresolution scanning electron microscope (HRSEM)
  • Negative staining
  • Surface

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

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