Scanning laser microscope able to detect the refraction of the laser beam

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have developed a scanning laser microscope system that can measure the spatial inhomogeneity of the refractive index using the laser beam refracted by the sample. Using this microscope, we can observe samples which are nearly transparent, such as live cells, without any use of dyes. Furthermore, we have visualized the Brownian motion of cell structures by detecting random fluctuations in the photoelectric current. This allows us to understand the rigidity of cell structures and the mobility within them.

Original languageEnglish
Pages (from-to)3279-3282
Number of pages4
JournalJapanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume44
Issue number5 A
DOIs
Publication statusPublished - 2005 May

Fingerprint

Refraction
Laser beams
refraction
Microscopes
microscopes
laser beams
Scanning
scanning
Lasers
Brownian movement
cells
Rigidity
lasers
Refractive index
Dyes
rigidity
inhomogeneity
dyes
refractivity

Keywords

  • Brownian motion
  • Cell
  • Laser microscope
  • Quadrant photodiodes
  • Refractive index

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

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abstract = "We have developed a scanning laser microscope system that can measure the spatial inhomogeneity of the refractive index using the laser beam refracted by the sample. Using this microscope, we can observe samples which are nearly transparent, such as live cells, without any use of dyes. Furthermore, we have visualized the Brownian motion of cell structures by detecting random fluctuations in the photoelectric current. This allows us to understand the rigidity of cell structures and the mobility within them.",
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AB - We have developed a scanning laser microscope system that can measure the spatial inhomogeneity of the refractive index using the laser beam refracted by the sample. Using this microscope, we can observe samples which are nearly transparent, such as live cells, without any use of dyes. Furthermore, we have visualized the Brownian motion of cell structures by detecting random fluctuations in the photoelectric current. This allows us to understand the rigidity of cell structures and the mobility within them.

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KW - Laser microscope

KW - Quadrant photodiodes

KW - Refractive index

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