Seasonal dominance of CL500-11 bacterioplankton (phylum Chloroflexi) in the oxygenated hypolimnion of Lake Biwa, Japan

Yusuke Okazaki, Yoshikuni Hodoki, Shin Ichi Nakano

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Uncultured bacteria affiliated with the CL500-11 cluster (phylum Chloroflexi) were first reported from the oxygenated hypolimnion of Crater Lake (USA) as a predominant bacterioplankton, although this dominance has not been reported in other environments. In this study, we showed that CL500-11 is also dominant in the oxygenated hypolimnion of Lake Biwa (Japan) and followed its spatiotemporal succession using fluorescent in situ hybridization. CL500-11 cells were almost absent [< 1% of 4′,6-diamidino-2-phenylindole (DAPI)-stained cells] at the beginning of the stratification period, dominated (> 10% of DAPI-stained cells; maximum = 16.5%) in the hypolimnion during the stratification period, and decreased to below the detection limit with the collapse of the thermocline. This pattern was observed over two annual cycles. A longitudinal assessment also showed that CL500-11 was the dominant bacterium in the hypolimnion over the whole lake, but was generally undetectable in the stratified epilimnion. These data suggest that CL500-11 is acclimated to the oxygenated hypolimnion and is a potentially important component of the pelagic biogeochemical cycling of the lake. A comparative analysis of 16S rRNA gene sequences revealed that almost all CL500-11 sequences previously deposited in the database were detected from hypolimnion or holomictic water in deep oxic freshwater lakes, suggesting that the bacteria may form one of the common lineages residing in an aerobic hypolimnetic niche.

Original languageEnglish
Pages (from-to)82-92
Number of pages11
JournalFEMS Microbiology Ecology
Volume83
Issue number1
DOIs
Publication statusPublished - 2013 Jan
Externally publishedYes

Fingerprint

Chloroflexi
hypolimnion
bacterioplankton
Lakes
Japan
lake
Bacteria
bacterium
crater lake
Fresh Water
epilimnion
Fluorescence In Situ Hybridization
rRNA Genes
Limit of Detection
thermocline
annual cycle
Databases
niche
stratification
Water

Keywords

  • Anaerolineae
  • CARD-FISH
  • Freshwater bacterioplankton

ASJC Scopus subject areas

  • Ecology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Seasonal dominance of CL500-11 bacterioplankton (phylum Chloroflexi) in the oxygenated hypolimnion of Lake Biwa, Japan. / Okazaki, Yusuke; Hodoki, Yoshikuni; Nakano, Shin Ichi.

In: FEMS Microbiology Ecology, Vol. 83, No. 1, 01.2013, p. 82-92.

Research output: Contribution to journalArticle

Okazaki, Yusuke ; Hodoki, Yoshikuni ; Nakano, Shin Ichi. / Seasonal dominance of CL500-11 bacterioplankton (phylum Chloroflexi) in the oxygenated hypolimnion of Lake Biwa, Japan. In: FEMS Microbiology Ecology. 2013 ; Vol. 83, No. 1. pp. 82-92.
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