Secretory granules of B-cells in the synovial membrane - An ultrastructural and cytochemical study

Y. Okada, I. Nakanishi, K. Kajikawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Ultrastructural and cytochemical studies have been made on secretory granules of B-cells (fibroblast-like cells) in the knee-joint synovium. The secretory granules were membrane-bounded spherical or slightly elongated bodies, 150 to 350 nm (average 230 nm) in diameter and had a homogenous matrix with several cores. These granules were found in B-cells of all animal species examined; they were numerous in mice and rats, and few in guinea pigs, rabbits and man. Ultrastructural and cytochemical examinations revealed that the Golgi apparatus was involved in the formation of the secretory granules. Unlike lysosomes, they showed no acid phosphatase activity. The granule matrix was positively stained by Thiéiy's periodic acid-thiocarbohydrazidesilver proteinate technique, and the cores were digested by protease. These findings suggest that the granule matrix contains mucopolysaccharide(s) and/or glycoprotein(s) and the core material is largely proteinaceous in nature.

Original languageEnglish
Pages (from-to)131-141
Number of pages11
JournalCell and Tissue Research
Volume216
Issue number1
DOIs
Publication statusPublished - 1981 Mar

Fingerprint

Synovial Membrane
Secretory Vesicles
B-Lymphocytes
Cells
Membranes
Periodic Acid
Fibroblasts
Acid Phosphatase
Glycosaminoglycans
Rats
Glycoproteins
Animals
Peptide Hydrolases
Golgi Apparatus
Knee Joint
Lysosomes
Guinea Pigs
Rabbits

Keywords

  • Cytochemistry
  • Secretory granules
  • Synovial membrane
  • Ultrastructure

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Secretory granules of B-cells in the synovial membrane - An ultrastructural and cytochemical study. / Okada, Y.; Nakanishi, I.; Kajikawa, K.

In: Cell and Tissue Research, Vol. 216, No. 1, 03.1981, p. 131-141.

Research output: Contribution to journalArticle

Okada, Y. ; Nakanishi, I. ; Kajikawa, K. / Secretory granules of B-cells in the synovial membrane - An ultrastructural and cytochemical study. In: Cell and Tissue Research. 1981 ; Vol. 216, No. 1. pp. 131-141.
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