Abstract
Ultrastructural and cytochemical studies have been made on secretory granules of B-cells (fibroblast-like cells) in the knee-joint synovium. The secretory granules were membrane-bounded spherical or slightly elongated bodies, 150 to 350 nm (average 230 nm) in diameter and had a homogenous matrix with several cores. These granules were found in B-cells of all animal species examined; they were numerous in mice and rats, and few in guinea pigs, rabbits and man. Ultrastructural and cytochemical examinations revealed that the Golgi apparatus was involved in the formation of the secretory granules. Unlike lysosomes, they showed no acid phosphatase activity. The granule matrix was positively stained by Thiéiy's periodic acid-thiocarbohydrazidesilver proteinate technique, and the cores were digested by protease. These findings suggest that the granule matrix contains mucopolysaccharide(s) and/or glycoprotein(s) and the core material is largely proteinaceous in nature.
| Original language | English |
|---|---|
| Pages (from-to) | 131-141 |
| Number of pages | 11 |
| Journal | Cell and Tissue Research |
| Volume | 216 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1981 Mar |
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Keywords
- Cytochemistry
- Secretory granules
- Synovial membrane
- Ultrastructure
ASJC Scopus subject areas
- Anatomy
- Clinical Biochemistry
- Cell Biology
Cite this
Secretory granules of B-cells in the synovial membrane - An ultrastructural and cytochemical study. / Okada, Y.; Nakanishi, I.; Kajikawa, K.
In: Cell and Tissue Research, Vol. 216, No. 1, 03.1981, p. 131-141.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Secretory granules of B-cells in the synovial membrane - An ultrastructural and cytochemical study
AU - Okada, Y.
AU - Nakanishi, I.
AU - Kajikawa, K.
PY - 1981/3
Y1 - 1981/3
N2 - Ultrastructural and cytochemical studies have been made on secretory granules of B-cells (fibroblast-like cells) in the knee-joint synovium. The secretory granules were membrane-bounded spherical or slightly elongated bodies, 150 to 350 nm (average 230 nm) in diameter and had a homogenous matrix with several cores. These granules were found in B-cells of all animal species examined; they were numerous in mice and rats, and few in guinea pigs, rabbits and man. Ultrastructural and cytochemical examinations revealed that the Golgi apparatus was involved in the formation of the secretory granules. Unlike lysosomes, they showed no acid phosphatase activity. The granule matrix was positively stained by Thiéiy's periodic acid-thiocarbohydrazidesilver proteinate technique, and the cores were digested by protease. These findings suggest that the granule matrix contains mucopolysaccharide(s) and/or glycoprotein(s) and the core material is largely proteinaceous in nature.
AB - Ultrastructural and cytochemical studies have been made on secretory granules of B-cells (fibroblast-like cells) in the knee-joint synovium. The secretory granules were membrane-bounded spherical or slightly elongated bodies, 150 to 350 nm (average 230 nm) in diameter and had a homogenous matrix with several cores. These granules were found in B-cells of all animal species examined; they were numerous in mice and rats, and few in guinea pigs, rabbits and man. Ultrastructural and cytochemical examinations revealed that the Golgi apparatus was involved in the formation of the secretory granules. Unlike lysosomes, they showed no acid phosphatase activity. The granule matrix was positively stained by Thiéiy's periodic acid-thiocarbohydrazidesilver proteinate technique, and the cores were digested by protease. These findings suggest that the granule matrix contains mucopolysaccharide(s) and/or glycoprotein(s) and the core material is largely proteinaceous in nature.
KW - Cytochemistry
KW - Secretory granules
KW - Synovial membrane
KW - Ultrastructure
UR - http://www.scopus.com/inward/record.url?scp=0019476437&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019476437&partnerID=8YFLogxK
U2 - 10.1007/BF00234549
DO - 10.1007/BF00234549
M3 - Article
C2 - 6164489
AN - SCOPUS:0019476437
VL - 216
SP - 131
EP - 141
JO - Cell and Tissue Research
JF - Cell and Tissue Research
SN - 0302-766X
IS - 1
ER -