Segregation and pathfinding of callosal axons through EphA3 signaling

Mitsuaki Nishikimi, Koji Oishi, Hidenori Tabata, Kenichi Torii, Kazunori Nakajima

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The corpus callosum, composed of callosal axons, is the largest structure among commissural connections in eutherian animals. Axon pathfinding of callosal neurons has been shown to be guided by intermediate targets, such as midline glial structures. However, it has not yet been understood completely how axon-axon interactions, another major mechanism for axon pathfinding, are involved in the pathfinding of callosal neurons. Here, we show that callosal axons from the medial and lateral regions of the mouse cerebral cortex pass through the dorsal and ventral parts, respectively, of the corpus callosum. Using an explant culture system, we observed that the axons from the medial and lateral cortices were segregated from each other in vitro, and that this segregation was attenuated by inhibition of EphA3 signaling.Wealso found that knockdown of EphA3, which is preferentially expressed in the lateral cortex, resulted in disorganized segregation of the callosal axons and disrupted axon pathfinding in vivo. These results together suggest the role of axonal segregation in the corpus callosum, mediated at least in part by EphA3, in correct pathfinding of callosal neurons.

Original languageEnglish
Pages (from-to)16251-16260
Number of pages10
JournalJournal of Neuroscience
Volume31
Issue number45
DOIs
Publication statusPublished - 2011 Nov 9

Fingerprint

Corpus Callosum
Axons
Neurons
Axon Guidance
Neuroglia
Cerebral Cortex

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Segregation and pathfinding of callosal axons through EphA3 signaling. / Nishikimi, Mitsuaki; Oishi, Koji; Tabata, Hidenori; Torii, Kenichi; Nakajima, Kazunori.

In: Journal of Neuroscience, Vol. 31, No. 45, 09.11.2011, p. 16251-16260.

Research output: Contribution to journalArticle

Nishikimi, Mitsuaki ; Oishi, Koji ; Tabata, Hidenori ; Torii, Kenichi ; Nakajima, Kazunori. / Segregation and pathfinding of callosal axons through EphA3 signaling. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 45. pp. 16251-16260.
@article{1848ee2b2dda4bc682fd4bad89db8f6c,
title = "Segregation and pathfinding of callosal axons through EphA3 signaling",
abstract = "The corpus callosum, composed of callosal axons, is the largest structure among commissural connections in eutherian animals. Axon pathfinding of callosal neurons has been shown to be guided by intermediate targets, such as midline glial structures. However, it has not yet been understood completely how axon-axon interactions, another major mechanism for axon pathfinding, are involved in the pathfinding of callosal neurons. Here, we show that callosal axons from the medial and lateral regions of the mouse cerebral cortex pass through the dorsal and ventral parts, respectively, of the corpus callosum. Using an explant culture system, we observed that the axons from the medial and lateral cortices were segregated from each other in vitro, and that this segregation was attenuated by inhibition of EphA3 signaling.Wealso found that knockdown of EphA3, which is preferentially expressed in the lateral cortex, resulted in disorganized segregation of the callosal axons and disrupted axon pathfinding in vivo. These results together suggest the role of axonal segregation in the corpus callosum, mediated at least in part by EphA3, in correct pathfinding of callosal neurons.",
author = "Mitsuaki Nishikimi and Koji Oishi and Hidenori Tabata and Kenichi Torii and Kazunori Nakajima",
year = "2011",
month = "11",
day = "9",
doi = "10.1523/JNEUROSCI.3303-11.2011",
language = "English",
volume = "31",
pages = "16251--16260",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "45",

}

TY - JOUR

T1 - Segregation and pathfinding of callosal axons through EphA3 signaling

AU - Nishikimi, Mitsuaki

AU - Oishi, Koji

AU - Tabata, Hidenori

AU - Torii, Kenichi

AU - Nakajima, Kazunori

PY - 2011/11/9

Y1 - 2011/11/9

N2 - The corpus callosum, composed of callosal axons, is the largest structure among commissural connections in eutherian animals. Axon pathfinding of callosal neurons has been shown to be guided by intermediate targets, such as midline glial structures. However, it has not yet been understood completely how axon-axon interactions, another major mechanism for axon pathfinding, are involved in the pathfinding of callosal neurons. Here, we show that callosal axons from the medial and lateral regions of the mouse cerebral cortex pass through the dorsal and ventral parts, respectively, of the corpus callosum. Using an explant culture system, we observed that the axons from the medial and lateral cortices were segregated from each other in vitro, and that this segregation was attenuated by inhibition of EphA3 signaling.Wealso found that knockdown of EphA3, which is preferentially expressed in the lateral cortex, resulted in disorganized segregation of the callosal axons and disrupted axon pathfinding in vivo. These results together suggest the role of axonal segregation in the corpus callosum, mediated at least in part by EphA3, in correct pathfinding of callosal neurons.

AB - The corpus callosum, composed of callosal axons, is the largest structure among commissural connections in eutherian animals. Axon pathfinding of callosal neurons has been shown to be guided by intermediate targets, such as midline glial structures. However, it has not yet been understood completely how axon-axon interactions, another major mechanism for axon pathfinding, are involved in the pathfinding of callosal neurons. Here, we show that callosal axons from the medial and lateral regions of the mouse cerebral cortex pass through the dorsal and ventral parts, respectively, of the corpus callosum. Using an explant culture system, we observed that the axons from the medial and lateral cortices were segregated from each other in vitro, and that this segregation was attenuated by inhibition of EphA3 signaling.Wealso found that knockdown of EphA3, which is preferentially expressed in the lateral cortex, resulted in disorganized segregation of the callosal axons and disrupted axon pathfinding in vivo. These results together suggest the role of axonal segregation in the corpus callosum, mediated at least in part by EphA3, in correct pathfinding of callosal neurons.

UR - http://www.scopus.com/inward/record.url?scp=80755143631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80755143631&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.3303-11.2011

DO - 10.1523/JNEUROSCI.3303-11.2011

M3 - Article

C2 - 22072676

AN - SCOPUS:80755143631

VL - 31

SP - 16251

EP - 16260

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 45

ER -