Selection of ganglioside GM1-binding peptides by using a phage library

Teruhiko Matsubara, Dai Ishikawa, Takao Taki, Yoshio Okahata, Toshinori Sato

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Ganglioside Galβ1→3GalNAcβ1→4(NeuAcα2→3)Galβ1→4Glcβ1→1'Cer (GM1)-binding peptides were obtained from a phage-displayed pentadecapeptide library by an affinity selection. The selection processes were in situ-monitored by a quartz-crystal microbalance method, on which a ganglioside GM1 monolayer was transferred. After five rounds of biopanning, the DNA sequencing of 18 selected phages showed that only three individual clones were selected. The peptide sequences of the random region were found to be DFRRLPGAFWQLRQP, GWWYKGRARPVSAVA and VWRLLAPPFSNRLLP. Binding constants of these phage clones to the GM1 monolayer were 1010 M-1. Three synthetic pentadecapeptides inhibited the binding of cholera toxin B subunit to the GM1 monolayer with an IC50 of 24, 13 and 1.0 μM, respectively. These peptides will be useful for searching functional roles of ganglioside GM1. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)253-256
Number of pages4
JournalFEBS Letters
Volume456
Issue number2
DOIs
Publication statusPublished - 1999 Aug 6
Externally publishedYes

Fingerprint

G(M1) Ganglioside
Bacteriophages
Monolayers
Peptides
Clone Cells
Quartz Crystal Microbalance Techniques
Gangliosides
Quartz crystal microbalances
Cholera Toxin
DNA Sequence Analysis
Inhibitory Concentration 50
Libraries
DNA

Keywords

  • Carbohydrate recognition
  • Galβ1→3GalNAcβ1→4(NeuAcα2→3)Galβ1→4Glcβ1→1'Cer
  • Ganglioside
  • Monolayer
  • Phage-displayed peptide library
  • Quartz-crystal microbalance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Selection of ganglioside GM1-binding peptides by using a phage library. / Matsubara, Teruhiko; Ishikawa, Dai; Taki, Takao; Okahata, Yoshio; Sato, Toshinori.

In: FEBS Letters, Vol. 456, No. 2, 06.08.1999, p. 253-256.

Research output: Contribution to journalArticle

Matsubara, Teruhiko ; Ishikawa, Dai ; Taki, Takao ; Okahata, Yoshio ; Sato, Toshinori. / Selection of ganglioside GM1-binding peptides by using a phage library. In: FEBS Letters. 1999 ; Vol. 456, No. 2. pp. 253-256.
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