Selection of peptides with the recognition of stereoregular PMMA from phage library

Toshiki Sawada, Hisao Matsuno, Teruhiko Matsubara, Toshinori Sato, Tatsuki Kitayama, Takeshi Serizawa

Research output: Contribution to conferencePaper

Abstract

To identify peptides with affinity selective for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), phage library displaying a heptamer random peptide was used. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. Binding affinity and specificity of these phage clones were quantitatively investigated by enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

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Keywords

  • Peptide
  • Phage library
  • Poly(methyl methacrylate)
  • Tacticity

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Sawada, T., Matsuno, H., Matsubara, T., Sato, T., Kitayama, T., & Serizawa, T. (2005). Selection of peptides with the recognition of stereoregular PMMA from phage library. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.