Selection of peptides with the recognition of stereoregular PMMA from phage library

Toshiki Sawada, Hisao Matsuno, Teruhiko Matsubara, Toshinori Sato, Tatsuki Kitayama, Takeshi Serizawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To identify peptides with affinity selective for stereoregular isotactic poly(methyl methacrylate) (it-PMMA), phage library displaying a heptamer random peptide was used. After five rounds of biopanning, several phage clones with high specific affinity to it-PMMA were selected. As a result of DNA sequencing, nine kinds of it-PMMA binding phage clones were isolated. Binding affinity and specificity of these phage clones were quantitatively investigated by enzyme-linked immunosorbent assay. These clones not only bound to it-PMMA greater than phage library, but showed the specific binding to it-PMMA rather than syndiotactic and atactic PMMAs.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages2081
Number of pages1
Volume54
Edition1
Publication statusPublished - 2005
Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
Duration: 2005 May 252005 May 27

Other

Other54th SPSJ Annual Meeting 2005
CountryJapan
CityYokohama
Period05/5/2505/5/27

Fingerprint

Bacteriophages
Polymethyl methacrylates
Peptides
Assays
DNA
Enzymes

Keywords

  • Peptide
  • Phage library
  • Poly(methyl methacrylate)
  • Tacticity

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Sawada, T., Matsuno, H., Matsubara, T., Sato, T., Kitayama, T., & Serizawa, T. (2005). Selection of peptides with the recognition of stereoregular PMMA from phage library. In Polymer Preprints, Japan (1 ed., Vol. 54, pp. 2081)

Selection of peptides with the recognition of stereoregular PMMA from phage library. / Sawada, Toshiki; Matsuno, Hisao; Matsubara, Teruhiko; Sato, Toshinori; Kitayama, Tatsuki; Serizawa, Takeshi.

Polymer Preprints, Japan. Vol. 54 1. ed. 2005. p. 2081.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sawada, T, Matsuno, H, Matsubara, T, Sato, T, Kitayama, T & Serizawa, T 2005, Selection of peptides with the recognition of stereoregular PMMA from phage library. in Polymer Preprints, Japan. 1 edn, vol. 54, pp. 2081, 54th SPSJ Annual Meeting 2005, Yokohama, Japan, 05/5/25.
Sawada T, Matsuno H, Matsubara T, Sato T, Kitayama T, Serizawa T. Selection of peptides with the recognition of stereoregular PMMA from phage library. In Polymer Preprints, Japan. 1 ed. Vol. 54. 2005. p. 2081
Sawada, Toshiki ; Matsuno, Hisao ; Matsubara, Teruhiko ; Sato, Toshinori ; Kitayama, Tatsuki ; Serizawa, Takeshi. / Selection of peptides with the recognition of stereoregular PMMA from phage library. Polymer Preprints, Japan. Vol. 54 1. ed. 2005. pp. 2081
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