Selective capture and collection of live target cells using a photoreactive silicon wafer device modified with antibodies via a photocleavable linker

Shinya Ariyasu, Kengo Hanaya, Eita Watanabe, Toshihiro Suzuki, Kazutaka Horie, Masanori Hayase, Ryo Abe, Shin Aoki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A device for the capture and recollection of live target cells is described. The platform was a silicon (Si) wafer modified with an anti-HEL antibody (anti-HEL-IgG, HEL = hen egg lysozyme) through a photocleavable 3-amino-3-(2-nitrophenyl)propionic acid (ANP) linker. The modification processes of the Si wafer surface were monitored by Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR) and fast-scanning atomic force microscopy (FS-AFM). The attachment of IgG and its release reaction on the Si surface via the photochemical cleavage of the ANP linker were observed directly by FS-AFM. The results of an enzyme-linked immunosorbent assay (ELISA) indicated that the photorelease of the complex of anti-HEL-IgG with the secondary antibody-alkaline phosphatase hybrid (secondary IgG-AP) from the Si surface occurs with minimum damage. Furthermore, it was possible to collect SP2/O cells selectively that express HEL on their cell membranes (SP2/O-HEL) on the Si wafer device. Photochemical cleavage of the ANP linker facilitated the effective release of living SP2/O cells whose viability was verified by staining experiments using tripan blue. Moreover, it was possible to reculture the recovered cells. This methodology represents an effective strategy for isolating intact target cells in the biological and medicinal sciences and related fields.

Original languageEnglish
Pages (from-to)13118-13126
Number of pages9
JournalLangmuir
Volume28
Issue number36
DOIs
Publication statusPublished - 2012 Sep 11
Externally publishedYes

Fingerprint

antibodies
Silicon wafers
Antibodies
Atrial Natriuretic Factor
Immunoglobulin G
wafers
Atomic force microscopy
silicon
Silicon
Enzymes
cells
Scanning
Propionic acid
Phosphatases
Cell membranes
cleavage
Immunosorbents
Fourier transform infrared spectroscopy
atomic force microscopy
Assays

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Selective capture and collection of live target cells using a photoreactive silicon wafer device modified with antibodies via a photocleavable linker. / Ariyasu, Shinya; Hanaya, Kengo; Watanabe, Eita; Suzuki, Toshihiro; Horie, Kazutaka; Hayase, Masanori; Abe, Ryo; Aoki, Shin.

In: Langmuir, Vol. 28, No. 36, 11.09.2012, p. 13118-13126.

Research output: Contribution to journalArticle

Ariyasu, Shinya ; Hanaya, Kengo ; Watanabe, Eita ; Suzuki, Toshihiro ; Horie, Kazutaka ; Hayase, Masanori ; Abe, Ryo ; Aoki, Shin. / Selective capture and collection of live target cells using a photoreactive silicon wafer device modified with antibodies via a photocleavable linker. In: Langmuir. 2012 ; Vol. 28, No. 36. pp. 13118-13126.
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