Selective contribution of IFN-α/β signaling to the maturation of dendritic cells induced by double-stranded RNA or viral infection

Kenya Honda, Shinya Sakaguchi, Chigusa Nakajima, Ai Watanabe, Hideyuki Yanai, Misako Matsumoto, Toshiaki Ohteki, Tsuneyasu Kaisho, Akinori Takaoka, Shizuo Akira, Tsukasa Seya, Tadatsugu Taniguchi

Research output: Contribution to journalArticle

300 Citations (Scopus)

Abstract

A complex mechanism may be operational for dendritic cell (DC) maturation, wherein Toll-like receptor and other signaling pathways may be coordinated differently depending on the nature of the pathogens, in order for DC maturation to be most effective to a given threat. Here, we show that IFN-α/β signaling is selectively required for the maturation of DCs induced by double-stranded RNA or viral infection in vitro. Interestingly, the maturation is still observed in the absence of either of the two target genes of IFN-α/β, TLR3 and PKR (double-stranded-RNA-dependent protein kinase R), indicating the complexity of the IFN-α/β-induced transcriptional program in DCs. We also show that the DCs stimulated in vivo by these agents can migrate into the T cell zone of the spleen but fail to mature without the IFN signal. The immune system may have acquired the selective utilization of this cytokine system, which is essential for innate antiviral immunity, to effectively couple with the induction of adaptive immunity.

Original languageEnglish
Pages (from-to)10872-10877
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number19
DOIs
Publication statusPublished - 2003 Sep 16
Externally publishedYes

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Double-Stranded RNA
Virus Diseases
Dendritic Cells
eIF-2 Kinase
Toll-Like Receptors
Adaptive Immunity
Innate Immunity
Antiviral Agents
Immune System
Spleen
Cytokines
T-Lymphocytes
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Selective contribution of IFN-α/β signaling to the maturation of dendritic cells induced by double-stranded RNA or viral infection. / Honda, Kenya; Sakaguchi, Shinya; Nakajima, Chigusa; Watanabe, Ai; Yanai, Hideyuki; Matsumoto, Misako; Ohteki, Toshiaki; Kaisho, Tsuneyasu; Takaoka, Akinori; Akira, Shizuo; Seya, Tsukasa; Taniguchi, Tadatsugu.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 19, 16.09.2003, p. 10872-10877.

Research output: Contribution to journalArticle

Honda, K, Sakaguchi, S, Nakajima, C, Watanabe, A, Yanai, H, Matsumoto, M, Ohteki, T, Kaisho, T, Takaoka, A, Akira, S, Seya, T & Taniguchi, T 2003, 'Selective contribution of IFN-α/β signaling to the maturation of dendritic cells induced by double-stranded RNA or viral infection', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 19, pp. 10872-10877. https://doi.org/10.1073/pnas.1934678100
Honda, Kenya ; Sakaguchi, Shinya ; Nakajima, Chigusa ; Watanabe, Ai ; Yanai, Hideyuki ; Matsumoto, Misako ; Ohteki, Toshiaki ; Kaisho, Tsuneyasu ; Takaoka, Akinori ; Akira, Shizuo ; Seya, Tsukasa ; Taniguchi, Tadatsugu. / Selective contribution of IFN-α/β signaling to the maturation of dendritic cells induced by double-stranded RNA or viral infection. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 19. pp. 10872-10877.
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