Selective elimination of messenger RNA prevents an incidence of untimely meiosis

Yuriko Harigaya, Hirotsugu Tanaka, Soichiro Yamanaka, Kayoko Tanaka, Yoshinori Watanabe, Chihiro Tsutsumi, Yuji Chikashige, Yasushi Hiraoka, Akira Yamashita, Masayuki Yamamoto

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Much remains unknown about the molecular regulation of meiosis. Here we show that meiosis-specific transcripts are selectively removed if expressed during vegetative growth in fission yeast. These messenger RNAs contain a cis-acting region - which we call the DSR - that confers this removal via binding to a YTH-family protein Mmi1. Loss of Mmi1 function severely impairs cell growth owing to the untimely expression of meiotic transcripts. Microarray analysis reveals that at least a dozen such meiosis-specific transcripts are eliminated by the DSR-Mmi1 system. Mmi1 remains in the form of multiple nuclear foci during vegetative growth. At meiotic prophase these foci precipitate to a single focus, which coincides with the dot formed by the master meiosis-regulator Mei2. A meiotic arrest due to the loss of the Mei2 dot is released by a reduction in Mmi1 activity. We propose that Mei2 turns off the DSR-Mmi1 system by sequestering Mmi1 to the dot and thereby secures stable expression of meiosis-specific transcripts.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalNature
Volume442
Issue number7098
DOIs
Publication statusPublished - 2006 Jul 6
Externally publishedYes

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Meiosis
Messenger RNA
Incidence
Growth
Prophase
Schizosaccharomyces
Microarray Analysis
Proteins

ASJC Scopus subject areas

  • General

Cite this

Harigaya, Y., Tanaka, H., Yamanaka, S., Tanaka, K., Watanabe, Y., Tsutsumi, C., ... Yamamoto, M. (2006). Selective elimination of messenger RNA prevents an incidence of untimely meiosis. Nature, 442(7098), 45-50. https://doi.org/10.1038/nature04881

Selective elimination of messenger RNA prevents an incidence of untimely meiosis. / Harigaya, Yuriko; Tanaka, Hirotsugu; Yamanaka, Soichiro; Tanaka, Kayoko; Watanabe, Yoshinori; Tsutsumi, Chihiro; Chikashige, Yuji; Hiraoka, Yasushi; Yamashita, Akira; Yamamoto, Masayuki.

In: Nature, Vol. 442, No. 7098, 06.07.2006, p. 45-50.

Research output: Contribution to journalArticle

Harigaya, Y, Tanaka, H, Yamanaka, S, Tanaka, K, Watanabe, Y, Tsutsumi, C, Chikashige, Y, Hiraoka, Y, Yamashita, A & Yamamoto, M 2006, 'Selective elimination of messenger RNA prevents an incidence of untimely meiosis', Nature, vol. 442, no. 7098, pp. 45-50. https://doi.org/10.1038/nature04881
Harigaya Y, Tanaka H, Yamanaka S, Tanaka K, Watanabe Y, Tsutsumi C et al. Selective elimination of messenger RNA prevents an incidence of untimely meiosis. Nature. 2006 Jul 6;442(7098):45-50. https://doi.org/10.1038/nature04881
Harigaya, Yuriko ; Tanaka, Hirotsugu ; Yamanaka, Soichiro ; Tanaka, Kayoko ; Watanabe, Yoshinori ; Tsutsumi, Chihiro ; Chikashige, Yuji ; Hiraoka, Yasushi ; Yamashita, Akira ; Yamamoto, Masayuki. / Selective elimination of messenger RNA prevents an incidence of untimely meiosis. In: Nature. 2006 ; Vol. 442, No. 7098. pp. 45-50.
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