Selective expansion of CD34+ cells from mouse bone marrow cultured on LH/P MP-coated plates with adequate cytokines

Satoko Kishimoto, Masayuki Ishihara, Yasuhiro Kanatani, Masaki Nambu, Megumi Takikawa, Yuki Sumi, Shingo Nakamura, Yasutaka Mori, Hidemi Hattori, Yoshihiro Tanaka, Toshinori Sato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Low-molecular-weight heparin/protamine microparticles (LH/P MPs) serve as carriers for controlled release of heparin-binding cytokines. LH/P MPs were stably coated onto plastic surfaces by drying. The purpose of this study is to evaluate a culture method for selective expansion of CD34+ cells using LH/P MPs as cytokine-binding matrix. Ficoll-purified mouse bone marrow cells (mouse FP-BMCs) containing CD34+ cells were cultured on LH/P MP-coated plates in the presence of stem cell factor (SCF), thrombopoietin (Tpo), and Flt-3 ligand (Flt-3) in hematopoietic progenitor growth medium (HPGM) supplemented with 4% heat-inactivated fetal bovine serum (FBS). After 8 days of culture, the total cell count increased 4.6-fold, and flow cytometry analyses revealed that 23.8% of the initial cells and 57.4% of the expanded cells were CD34 positive. Therefore, CD34+ cells were estimated to have increased 11.0-fold. In contrast, cultured CD34+ cells on uncoated tissue culture plates increased 5.8-fold in an identical medium.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of Tissue Engineering
Volume2
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

Protamines
Low Molecular Weight Heparin
Bone Marrow Cells
Bone
Molecular weight
Cytokines
Cultured Cells
Thrombopoietin
Ficoll
Tissue culture
Stem Cell Factor
Flow cytometry
Plastics
Heparin
Drying
Flow Cytometry
Stem cells
Cell Count
Hot Temperature
Cells

Keywords

  • CD34-positive hematopoietic cells (CD34 cells)
  • Cell proliferation
  • Cytokines

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Selective expansion of CD34+ cells from mouse bone marrow cultured on LH/P MP-coated plates with adequate cytokines. / Kishimoto, Satoko; Ishihara, Masayuki; Kanatani, Yasuhiro; Nambu, Masaki; Takikawa, Megumi; Sumi, Yuki; Nakamura, Shingo; Mori, Yasutaka; Hattori, Hidemi; Tanaka, Yoshihiro; Sato, Toshinori.

In: Journal of Tissue Engineering, Vol. 2, No. 1, 2011, p. 1-8.

Research output: Contribution to journalArticle

Kishimoto, S, Ishihara, M, Kanatani, Y, Nambu, M, Takikawa, M, Sumi, Y, Nakamura, S, Mori, Y, Hattori, H, Tanaka, Y & Sato, T 2011, 'Selective expansion of CD34+ cells from mouse bone marrow cultured on LH/P MP-coated plates with adequate cytokines', Journal of Tissue Engineering, vol. 2, no. 1, pp. 1-8. https://doi.org/10.1177/2041731411425419
Kishimoto, Satoko ; Ishihara, Masayuki ; Kanatani, Yasuhiro ; Nambu, Masaki ; Takikawa, Megumi ; Sumi, Yuki ; Nakamura, Shingo ; Mori, Yasutaka ; Hattori, Hidemi ; Tanaka, Yoshihiro ; Sato, Toshinori. / Selective expansion of CD34+ cells from mouse bone marrow cultured on LH/P MP-coated plates with adequate cytokines. In: Journal of Tissue Engineering. 2011 ; Vol. 2, No. 1. pp. 1-8.
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