Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue

Takahiko Shimizu; Keiichi Hamada; Haruhiko Isomura; Yuki Myotoishi; Susumu Ikegami; Hiroyuki Kaneko; Marina Dan-Sohkawa

doi:10.1016/0014-5793(95)00751-T

Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue

Takahiko Shimizu, Keiichi Hamada, Haruhiko Isomura, Yuki Myotoishi, Susumu Ikegami, Hiroyuki Kaneko, Marina Dan-Sohkawa

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

External application of 0.2-100 μg/ml albuside B inhibits gastrulation of the starfish (Asterina pectinifera) embryo. Treated embryos retain the late blastula morphology with the vegetal plate. However, the vegetal plate is unreactive to soybean agglutinin, a probe for observing the progenitor cells of the archenteron (mesendoderm) in a normal embryo. The effective period of the treatment is limited from 4 to 6 h after fertilization, a period immediately before the onset of blastulation. RNA synthesis is unaffected during the period of sensitivity. The selectivity of the inhibition shows that albuside B may be a useful tool for studying the mechanisms of mesendoderm differentiation.

Original language	English
Pages (from-to)	221-224
Number of pages	4
Journal	FEBS Letters
Volume	369
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(95)00751-T
Publication status	Published - 1995 Aug 7
Externally published	Yes

Keywords

Albuside B
Asterina pectinifera
Gastrula
Mesendoderm
Soybean agglutinin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)00751-T

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title = "Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue",

abstract = "External application of 0.2-100 μg/ml albuside B inhibits gastrulation of the starfish (Asterina pectinifera) embryo. Treated embryos retain the late blastula morphology with the vegetal plate. However, the vegetal plate is unreactive to soybean agglutinin, a probe for observing the progenitor cells of the archenteron (mesendoderm) in a normal embryo. The effective period of the treatment is limited from 4 to 6 h after fertilization, a period immediately before the onset of blastulation. RNA synthesis is unaffected during the period of sensitivity. The selectivity of the inhibition shows that albuside B may be a useful tool for studying the mechanisms of mesendoderm differentiation.",

keywords = "Albuside B, Asterina pectinifera, Gastrula, Mesendoderm, Soybean agglutinin",

author = "Takahiko Shimizu and Keiichi Hamada and Haruhiko Isomura and Yuki Myotoishi and Susumu Ikegami and Hiroyuki Kaneko and Marina Dan-Sohkawa",

note = "Funding Information: Acknowledgements.{"} We thank Hiroki Kobayashi for technical assistance. This study was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.",

year = "1995",

month = aug,

day = "7",

doi = "10.1016/0014-5793(95)00751-T",

language = "English",

volume = "369",

pages = "221--224",

journal = "FEBS Letters",

issn = "0014-5793",

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TY - JOUR

T1 - Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue

AU - Shimizu, Takahiko

AU - Hamada, Keiichi

AU - Isomura, Haruhiko

AU - Myotoishi, Yuki

AU - Ikegami, Susumu

AU - Kaneko, Hiroyuki

AU - Dan-Sohkawa, Marina

N1 - Funding Information: Acknowledgements." We thank Hiroki Kobayashi for technical assistance. This study was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.

PY - 1995/8/7

Y1 - 1995/8/7

N2 - External application of 0.2-100 μg/ml albuside B inhibits gastrulation of the starfish (Asterina pectinifera) embryo. Treated embryos retain the late blastula morphology with the vegetal plate. However, the vegetal plate is unreactive to soybean agglutinin, a probe for observing the progenitor cells of the archenteron (mesendoderm) in a normal embryo. The effective period of the treatment is limited from 4 to 6 h after fertilization, a period immediately before the onset of blastulation. RNA synthesis is unaffected during the period of sensitivity. The selectivity of the inhibition shows that albuside B may be a useful tool for studying the mechanisms of mesendoderm differentiation.

AB - External application of 0.2-100 μg/ml albuside B inhibits gastrulation of the starfish (Asterina pectinifera) embryo. Treated embryos retain the late blastula morphology with the vegetal plate. However, the vegetal plate is unreactive to soybean agglutinin, a probe for observing the progenitor cells of the archenteron (mesendoderm) in a normal embryo. The effective period of the treatment is limited from 4 to 6 h after fertilization, a period immediately before the onset of blastulation. RNA synthesis is unaffected during the period of sensitivity. The selectivity of the inhibition shows that albuside B may be a useful tool for studying the mechanisms of mesendoderm differentiation.

KW - Albuside B

KW - Asterina pectinifera

KW - Gastrula

KW - Mesendoderm

KW - Soybean agglutinin

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DO - 10.1016/0014-5793(95)00751-T

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AN - SCOPUS:0029123227

SN - 0014-5793

VL - 369

SP - 221

EP - 224

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue

Abstract

Keywords

ASJC Scopus subject areas

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