Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue

Takahiko Shimizu, Keiichi Hamada, Haruhiko Isomura, Yuki Myotoishi, Susumu Ikegami, Hiroyuki Kaneko, Marina Dan-Sohkawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

External application of 0.2-100 μg/ml albuside B inhibits gastrulation of the starfish (Asterina pectinifera) embryo. Treated embryos retain the late blastula morphology with the vegetal plate. However, the vegetal plate is unreactive to soybean agglutinin, a probe for observing the progenitor cells of the archenteron (mesendoderm) in a normal embryo. The effective period of the treatment is limited from 4 to 6 h after fertilization, a period immediately before the onset of blastulation. RNA synthesis is unaffected during the period of sensitivity. The selectivity of the inhibition shows that albuside B may be a useful tool for studying the mechanisms of mesendoderm differentiation.

Original languageEnglish
Pages (from-to)221-224
Number of pages4
JournalFEBS Letters
Volume369
Issue number2-3
DOIs
Publication statusPublished - 1995 Aug 7
Externally publishedYes

Fingerprint

Starfish
Inosine
Gastrulation
Embryonic Structures
Asterina
Blastula
Gastrula
RNA
Fertilization
Stem Cells
albuside B
soybean lectin

Keywords

  • Albuside B
  • Asterina pectinifera
  • Gastrula
  • Mesendoderm
  • Soybean agglutinin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Shimizu, T., Hamada, K., Isomura, H., Myotoishi, Y., Ikegami, S., Kaneko, H., & Dan-Sohkawa, M. (1995). Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue. FEBS Letters, 369(2-3), 221-224. https://doi.org/10.1016/0014-5793(95)00751-T

Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue. / Shimizu, Takahiko; Hamada, Keiichi; Isomura, Haruhiko; Myotoishi, Yuki; Ikegami, Susumu; Kaneko, Hiroyuki; Dan-Sohkawa, Marina.

In: FEBS Letters, Vol. 369, No. 2-3, 07.08.1995, p. 221-224.

Research output: Contribution to journalArticle

Shimizu, T, Hamada, K, Isomura, H, Myotoishi, Y, Ikegami, S, Kaneko, H & Dan-Sohkawa, M 1995, 'Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue', FEBS Letters, vol. 369, no. 2-3, pp. 221-224. https://doi.org/10.1016/0014-5793(95)00751-T
Shimizu, Takahiko ; Hamada, Keiichi ; Isomura, Haruhiko ; Myotoishi, Yuki ; Ikegami, Susumu ; Kaneko, Hiroyuki ; Dan-Sohkawa, Marina. / Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue. In: FEBS Letters. 1995 ; Vol. 369, No. 2-3. pp. 221-224.
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