Selective isolation of DNA or RNA using single-stranded DNA affinity latex particles

Takeshi Imai, Yoshiteru Sumi, Mamoru Hatakeyama, Keiji Fujimoto, Haruma Kawaguchi, Nobuaki Hayashida, Kazuo Shiozaki, Kunihiko Terada, Hiroaki Yajima, Hiroshi Handa

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We developed latex particles with a diameter of about 0.22 μm on which single-stranded (ss) DNA was covalently coupled to select or enrich its complementary DNA or mRNA. DNA was first covalently coupled to the latex particles in the double-stranded (ds) form with both blunt and protruding ends. More than 80% of the dsDNA was coupled through the ssDNA stretch at its protruding end. The presence of NaCl in the immobilization reaction severely inhibited DNA from coupling to the particles. The particles were then treated with alkali or heated to denature the dsDNA and sedimented by a brief centrifugation to yield ssDNA immobilized particles. They allowed the selective and efficient isolation of a desired RNA from total cellular RNA.

Original languageEnglish
Pages (from-to)245-249
Number of pages5
JournalJournal of Colloid And Interface Science
Volume177
Issue number1
DOIs
Publication statusPublished - 1996 Jan 15

Keywords

  • affinity latex particles
  • complementary RNA
  • hybridization selection
  • single-stranded DNA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

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  • Cite this

    Imai, T., Sumi, Y., Hatakeyama, M., Fujimoto, K., Kawaguchi, H., Hayashida, N., Shiozaki, K., Terada, K., Yajima, H., & Handa, H. (1996). Selective isolation of DNA or RNA using single-stranded DNA affinity latex particles. Journal of Colloid And Interface Science, 177(1), 245-249. https://doi.org/10.1006/jcis.1996.0027