Selective loss of gastrointestinal mast cells and impaired immunity in P13K-deficient mice

Taro Fukao, Taketo Yamada, Masanobu Tanabe, Yasuo Terauchi, Takayuki Ota, Tetsuro Takayama, Tomoichiro Asano, Tsutomu Takeuchi, Takashi Kadowaki, Jun Ichi Hata, Shigeo Koyasu

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Mice that lack the p85α regulatory subunit of phosphatidylinositol-3 kinase (P13K) are deficient in gastrointestinal and peritoneal mast cells but have dermal mast cells. Accordingly, these mice show impaired bacterial clearance in response to acute septic peritonitis and are highly susceptible to infection by the intestinal nematode Strongyloides venezuelensis. Systemic anaphylactic shock responses, however, are intact. We found that although reconstitution of P13K-/- mice with bone marrow-derived mast cells (BMMCs) restored anti-bacterial immunity, only T helper type 2 (TH2)-conditioned BMMCs, not "standard" BMMCs, were able to restore anti-nematode immunity. This finding highlights the importance of the TH2 response in the control of nematode infection. Thus, P13K likely plays an essential role in host immune responses by regulating both the development and induction of mast cells.

Original languageEnglish
Pages (from-to)295-304
Number of pages10
JournalNature Immunology
Volume3
Issue number3
DOIs
Publication statusPublished - 2002

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Mast Cells
Immunity
Nematode Infections
Bone Marrow
Phosphatidylinositol 3-Kinase
Strongyloides
Anaphylaxis
Peritonitis
Skin

ASJC Scopus subject areas

  • Immunology

Cite this

Fukao, T., Yamada, T., Tanabe, M., Terauchi, Y., Ota, T., Takayama, T., ... Koyasu, S. (2002). Selective loss of gastrointestinal mast cells and impaired immunity in P13K-deficient mice. Nature Immunology, 3(3), 295-304. https://doi.org/10.1038/ni768

Selective loss of gastrointestinal mast cells and impaired immunity in P13K-deficient mice. / Fukao, Taro; Yamada, Taketo; Tanabe, Masanobu; Terauchi, Yasuo; Ota, Takayuki; Takayama, Tetsuro; Asano, Tomoichiro; Takeuchi, Tsutomu; Kadowaki, Takashi; Hata, Jun Ichi; Koyasu, Shigeo.

In: Nature Immunology, Vol. 3, No. 3, 2002, p. 295-304.

Research output: Contribution to journalArticle

Fukao, T, Yamada, T, Tanabe, M, Terauchi, Y, Ota, T, Takayama, T, Asano, T, Takeuchi, T, Kadowaki, T, Hata, JI & Koyasu, S 2002, 'Selective loss of gastrointestinal mast cells and impaired immunity in P13K-deficient mice', Nature Immunology, vol. 3, no. 3, pp. 295-304. https://doi.org/10.1038/ni768
Fukao, Taro ; Yamada, Taketo ; Tanabe, Masanobu ; Terauchi, Yasuo ; Ota, Takayuki ; Takayama, Tetsuro ; Asano, Tomoichiro ; Takeuchi, Tsutomu ; Kadowaki, Takashi ; Hata, Jun Ichi ; Koyasu, Shigeo. / Selective loss of gastrointestinal mast cells and impaired immunity in P13K-deficient mice. In: Nature Immunology. 2002 ; Vol. 3, No. 3. pp. 295-304.
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