Selective metal binding by Vanabin2 from the vanadium-rich ascidian, Ascidia sydneiensis samea

Norifumi Kawakami, Tatsuya Ueki, Koichi Matsuo, Kunihiko Gekko, Hitoshi Michibata

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Vanadium-binding proteins, or Vanabins, have recently been isolated from the vanadium-rich ascidian, Ascidia sydneiensis samea. Recent reports indicate that Vanabin2 binds twenty V(IV) ions at pH 7.5, and that it has a novel bow-shaped conformation. However, the role of Vanabin2 in vanadium accumulation by the ascidian has not yet been determined. In the present study, the effects of acidic pH on selective metal binding to Vanabin2 and on the secondary structure of Vanabin2 were examined. Vanabin2 selectively bound to V(IV), Fe(III), and Cu(II) ions under acidic conditions. In contrast, Co(II), Ni(II), and Zn(II) ions were bound at pH 6.5 but not at pH 4.5. Changes in pH had no detectable effect on the secondary structure of Vanabin2 under acidic conditions, as determined by circular dichroism spectroscopy, and little variation in the dissociation constant for V(IV) ions was observed in the pH range 4.5-7.5, suggesting that the binding state of the ligands is not affected by acidification. Taken together, these results suggest that the reason for metal ion dissociation upon acidification is attributable not to a change in secondary structure but, rather, that it is caused by protonation of the amino acid ligands that complex with V(IV) ions.

Original languageEnglish
Pages (from-to)1096-1101
Number of pages6
JournalBiochimica et Biophysica Acta - General Subjects
Volume1760
Issue number7
DOIs
Publication statusPublished - 2006 Jul 1
Externally publishedYes

Fingerprint

Urochordata
Vanadium
Metals
Ions
Acidification
Circular dichroism spectroscopy
Ligands
Protonation
Metal ions
Conformations
Circular Dichroism
Carrier Proteins
Spectrum Analysis
Amino Acids

Keywords

  • Ascidian
  • Copper
  • Metal selectivity
  • Metal-binding protein
  • Vanadium

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Selective metal binding by Vanabin2 from the vanadium-rich ascidian, Ascidia sydneiensis samea. / Kawakami, Norifumi; Ueki, Tatsuya; Matsuo, Koichi; Gekko, Kunihiko; Michibata, Hitoshi.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1760, No. 7, 01.07.2006, p. 1096-1101.

Research output: Contribution to journalArticle

Kawakami, Norifumi ; Ueki, Tatsuya ; Matsuo, Koichi ; Gekko, Kunihiko ; Michibata, Hitoshi. / Selective metal binding by Vanabin2 from the vanadium-rich ascidian, Ascidia sydneiensis samea. In: Biochimica et Biophysica Acta - General Subjects. 2006 ; Vol. 1760, No. 7. pp. 1096-1101.
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