Selective proliferation of human γδ T cells in vitro

Song Hua Chen, Akinori Oki, Tadao Ohno, Satoko Ishikawa, Masataka Mochizuki, Yu Fang Che, Hui Ming Dai, Xi Rui Ge

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Abstract

The effect of monoethylphosphate (MEP, commercial available or synthesized) together with IL-2 on the selective proliferation of human γδ T cells in vitro from peripheral blood mononuclear cells (PBMC) of healthy donors and of cancer patients was investigated. The γδ T cells were stimulated by MEP to proliferate in a dose-dependent manner. The effect of synthesized MEP was 10 times greater than that of commercial MEP. When the PBMCs of healthy donors were cultured for 25 d in the medium containing different concentrations of MEP, the total cell number increased about 1000-3000 fold; and the ratio of γδ T cells reached to 70-80%. The selective expansion of γδ T cells depended on the synergic action of MEP and IL-2. The bulk cultured γδ T cells exhibited obvious cytotoxic activities against allogenic tumor cell lines (SQ-5, K562 and Daudi) and autologous tumor cells. The culture system described here not only offers a simple method for obtaining a large number of γδ T cells which may become a new effector in the adoptive immunotherapy, but also provides a useful model for the further studies of the structure and function of γδ T cells in vitro.

Original languageEnglish
Pages (from-to)177-187
Number of pages11
JournalCell Research
Volume6
Issue number2
Publication statusPublished - 1996

Fingerprint

T-Lymphocytes
Interleukin-2
Tissue Donors
Adoptive Immunotherapy
In Vitro Techniques
Tumor Cell Line
Cultured Cells
Blood Cells
Neoplasms
Cell Count

Keywords

  • γδ T cells proliferation
  • Cytotoxicity
  • Monoethylphosphate

ASJC Scopus subject areas

  • Cell Biology

Cite this

Chen, S. H., Oki, A., Ohno, T., Ishikawa, S., Mochizuki, M., Che, Y. F., ... Ge, X. R. (1996). Selective proliferation of human γδ T cells in vitro. Cell Research, 6(2), 177-187.

Selective proliferation of human γδ T cells in vitro. / Chen, Song Hua; Oki, Akinori; Ohno, Tadao; Ishikawa, Satoko; Mochizuki, Masataka; Che, Yu Fang; Dai, Hui Ming; Ge, Xi Rui.

In: Cell Research, Vol. 6, No. 2, 1996, p. 177-187.

Research output: Contribution to journalArticle

Chen, SH, Oki, A, Ohno, T, Ishikawa, S, Mochizuki, M, Che, YF, Dai, HM & Ge, XR 1996, 'Selective proliferation of human γδ T cells in vitro', Cell Research, vol. 6, no. 2, pp. 177-187.
Chen SH, Oki A, Ohno T, Ishikawa S, Mochizuki M, Che YF et al. Selective proliferation of human γδ T cells in vitro. Cell Research. 1996;6(2):177-187.
Chen, Song Hua ; Oki, Akinori ; Ohno, Tadao ; Ishikawa, Satoko ; Mochizuki, Masataka ; Che, Yu Fang ; Dai, Hui Ming ; Ge, Xi Rui. / Selective proliferation of human γδ T cells in vitro. In: Cell Research. 1996 ; Vol. 6, No. 2. pp. 177-187.
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