Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals

Akihiro Higuchi, Hiroyoshi Inoue, Yoshio Kaneko, Erina Oonishi, Kazuo Tsubota

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The ocular surface is strongly affected by oxidative stress, which causes many ocular diseases including dry eye. Previously, we showed that selenium compounds, e.g., selenoprotein P and Se-lactoferrin, were candidates for treatment of dry eye. This paper shows the efficacy of Se-lactoferrin for the treatment of dry eye compared with Diquas as a control drug using two dry eye models and incorporation of lactoferrin into corneal epithelial cells via lactoferrin receptors. We show the efficacy of Se-lactoferrin eye drops in the tobacco smoke exposure rat dry eye model and short-term rabbit dry eye model, although Diquas eye drops were only effective in the short-term rabbit dry eye model. These results indicate that Se-lactoferrin was useful in the oxidative stress-causing dry eye model. Se-lactoferrin was taken into corneal epithelium cells via lactoferrin receptors. We identified LRP1 as the lactoferrin receptor in the corneal epithelium involved in lactoferrin uptake. Se-lactoferrin eye drops did not irritate the ocular surface of rabbits. Se-lactoferrin was an excellent candidate for treatment of dry eye, reducing oxidative stress by a novel mechanism.

Original languageEnglish
Article number36903
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Nov 11

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Lactoferrin
Selenium
Animal Models
Epithelial Cells
Ophthalmic Solutions
Corneal Epithelium
Oxidative Stress
Rabbits
Selenoprotein P
Corneal Injuries
Selenium Compounds
Eye Diseases
Drug and Narcotic Control
Smoke
Tobacco

ASJC Scopus subject areas

  • General

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Selenium-binding lactoferrin is taken into corneal epithelial cells by a receptor and prevents corneal damage in dry eye model animals. / Higuchi, Akihiro; Inoue, Hiroyoshi; Kaneko, Yoshio; Oonishi, Erina; Tsubota, Kazuo.

In: Scientific Reports, Vol. 6, 36903, 11.11.2016.

Research output: Contribution to journalArticle

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