Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

Amy Y. Hsiao, Teru Okitsu, Hiroaki Onoe, Mahiro Kiyosawa, Hiroki Teramae, Shintaroh Iwanaga, Shigenori Miura, Tomohiko Kazama, Taro Matsumoto, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

Original languageEnglish
Title of host publicationIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Pages71-73
Number of pages3
DOIs
Publication statusPublished - 2013 Apr 2
Externally publishedYes
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Hsiao, A. Y., Okitsu, T., Onoe, H., Kiyosawa, M., Teramae, H., Iwanaga, S., Miura, S., Kazama, T., Matsumoto, T., & Takeuchi, S. (2013). Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 71-73). [6474179] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474179