Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

Amy Y. Hsiao, Teru Okitsu, Hiroaki Onoe, Mahiro Kiyosawa, Hiroki Teramae, Shintaroh Iwanaga, Shigenori Miura, Tomohiko Kazama, Taro Matsumoto, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages71-73
Number of pages3
DOIs
Publication statusPublished - 2013
Externally publishedYes
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

Fingerprint

smooth muscle
muscle cells
Oils and fats
Self assembly
fats
Muscle
self assembly
Fats
cells
Fibers
fibers
Female Contraceptive Devices
fibrin
Alginate
microfibers
Fibrin
Microfluidics
Collagen
collagens
stimulation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Hsiao, A. Y., Okitsu, T., Onoe, H., Kiyosawa, M., Teramae, H., Iwanaga, S., ... Takeuchi, S. (2013). Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 71-73). [6474179] https://doi.org/10.1109/MEMSYS.2013.6474179

Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. / Hsiao, Amy Y.; Okitsu, Teru; Onoe, Hiroaki; Kiyosawa, Mahiro; Teramae, Hiroki; Iwanaga, Shintaroh; Miura, Shigenori; Kazama, Tomohiko; Matsumoto, Taro; Takeuchi, Shoji.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 71-73 6474179.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hsiao, AY, Okitsu, T, Onoe, H, Kiyosawa, M, Teramae, H, Iwanaga, S, Miura, S, Kazama, T, Matsumoto, T & Takeuchi, S 2013, Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6474179, pp. 71-73, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 13/1/20. https://doi.org/10.1109/MEMSYS.2013.6474179
Hsiao AY, Okitsu T, Onoe H, Kiyosawa M, Teramae H, Iwanaga S et al. Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 71-73. 6474179 https://doi.org/10.1109/MEMSYS.2013.6474179
Hsiao, Amy Y. ; Okitsu, Teru ; Onoe, Hiroaki ; Kiyosawa, Mahiro ; Teramae, Hiroki ; Iwanaga, Shintaroh ; Miura, Shigenori ; Kazama, Tomohiko ; Matsumoto, Taro ; Takeuchi, Shoji. / Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. pp. 71-73
@inproceedings{9b7bd4774591471a9a311b65e9a8d677,
title = "Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells",
abstract = "This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.",
author = "Hsiao, {Amy Y.} and Teru Okitsu and Hiroaki Onoe and Mahiro Kiyosawa and Hiroki Teramae and Shintaroh Iwanaga and Shigenori Miura and Tomohiko Kazama and Taro Matsumoto and Shoji Takeuchi",
year = "2013",
doi = "10.1109/MEMSYS.2013.6474179",
language = "English",
isbn = "9781467356558",
pages = "71--73",
booktitle = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

}

TY - GEN

T1 - Self-assembly of cell springs using smooth muscle-like cells differentiated from multipotent cells

AU - Hsiao, Amy Y.

AU - Okitsu, Teru

AU - Onoe, Hiroaki

AU - Kiyosawa, Mahiro

AU - Teramae, Hiroki

AU - Iwanaga, Shintaroh

AU - Miura, Shigenori

AU - Kazama, Tomohiko

AU - Matsumoto, Taro

AU - Takeuchi, Shoji

PY - 2013

Y1 - 2013

N2 - This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

AB - This paper describes the self-assembly of 'cell springs' using smooth muscle-like cells differentiated from multipotent dedifferentiated fat (DFAT) cells. Using a microfluidic co-axial device, DFAT cells suspended in extracellular matrix (ECM) mixture of fibrin and collagen were encapsulated within tubular alginate microfibers and formed into 3D fiber-shaped micro-constructs. Such DFAT cell fibers were then induced to differentiate into smooth muscle cells (SMCs) under various conditions. Upon differentiation induction, DFAT cell fibers spontaneously contracted into coiled spring structures within 3 days. After 3 weeks of differentiation, the differentiated DFAT cell fibers showed synchronized contraction upon electrical stimulation.

UR - http://www.scopus.com/inward/record.url?scp=84875435888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875435888&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2013.6474179

DO - 10.1109/MEMSYS.2013.6474179

M3 - Conference contribution

SN - 9781467356558

SP - 71

EP - 73

BT - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

ER -