Sentan: A novel specific component of the apical structure of vertebrate motile cilia

Akiharu Kubo, Akiko Kubo, Sachiko Tsukita, Shoichiro Tsukita, Masayuki Amagai

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Human respiratory and oviductal cilia have specific apical structures characterized by a narrowed distal portion and a ciliary crown. These structures are conserved among vertebrates that have air respiration systems; however, the molecular components of these structures have not been defined, and their functions are unknown. To identify the molecular component(s) of the cilia apical structure, we screened EST libraries to identify gene(s) that are exclusively expressed in ciliated tissues, are transcriptionally up-regulated during in vitro ciliogenesis, and are not expressed in testis (because sperm flagella have no such apical structures). One of the identified gene products, named sentan, was localized to the distal tip region of motile cilia. Using anti-sentan polyclonal antibodies and electron microscopy, sentan was shown to localize exclusively to the bridging structure between the cell membrane and peripheral singlet microtubules, which specifically exists in the narrowed distal portion of cilia. Exogenously expressed sentan showed affinity for the membrane protrusions, and a protein-lipid binding assay revealed that sentan bound to phosphatidylserine. These findings suggest that sentan is the first molecular component of the ciliary tip to bridge the cell membrane and peripheral singlet microtubules, making the distal portion of the cilia narrow and stiff to allow for better airway clearance or ovum transport.

Original languageEnglish
Pages (from-to)5338-5346
Number of pages9
JournalMolecular Biology of the Cell
Volume19
Issue number12
DOIs
Publication statusPublished - 2008 Dec

Fingerprint

Cilia
Vertebrates
Microtubules
Ovum Transport
Cell Membrane Structures
Sperm Tail
Phosphatidylserines
Expressed Sequence Tags
Molecular Structure
Crowns
Protein Binding
Genes
Testis
Electron Microscopy
Respiration
Air
Cell Membrane
Lipids
Membranes
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Sentan : A novel specific component of the apical structure of vertebrate motile cilia. / Kubo, Akiharu; Kubo, Akiko; Tsukita, Sachiko; Tsukita, Shoichiro; Amagai, Masayuki.

In: Molecular Biology of the Cell, Vol. 19, No. 12, 12.2008, p. 5338-5346.

Research output: Contribution to journalArticle

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