Separation-Based Enzymomics Assay for the Discovery of Altered Peptide-Metabolizing Enzymatic Activities in Biosamples

Yuki Ichihashi, Toru Komatsu, Etsu Kyo, Hiroyuki Matsuzaki, Keisuke Hata, Toshiaki Watanabe, Tasuku Ueno, Kenjiro Hanaoka, Yasuteru Urano

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We have developed a novel method to globally monitor the enzymatic activities of biological samples based on performing the global activity analysis on a proteome separated by native electrophoresis. The study of the alteration in peptide-metabolizing enzymatic activity in colorectal tumor specimens led us to the discovery of elevated thimet oligopeptidase activity, which contributed to the faster consumption of immune-stimulating peptide neurotensin.

Original languageEnglish
Pages (from-to)11497-11501
Number of pages5
JournalAnalytical Chemistry
Volume91
Issue number18
DOIs
Publication statusPublished - 2019 Sep 17
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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