TY - JOUR
T1 - Separation-Based Enzymomics Assay for the Discovery of Altered Peptide-Metabolizing Enzymatic Activities in Biosamples
AU - Ichihashi, Yuki
AU - Komatsu, Toru
AU - Kyo, Etsu
AU - Matsuzaki, Hiroyuki
AU - Hata, Keisuke
AU - Watanabe, Toshiaki
AU - Ueno, Tasuku
AU - Hanaoka, Kenjiro
AU - Urano, Yasuteru
N1 - Funding Information:
This work was financially supported by MEXT (24655147 15H05371, 15K14937, 17K19477, 18H04538, and 19H02846 to T.K. and 16H05099 and 18H04609 to K.Hanaoka.), JST (T.K. and K.Hanaoka.), and AMED (Y.U.). T.K. was supported by The Naito Foundation and The Mochida Memorial Foundation for Medical and Pharmaceutical Research and The Tokyo Biochemical Research Foundation. PMF analysis was performed as a contract service by Apro Science. We would like to thank Gilles-Olivier Gratien for constructive comments on the manuscript.
Funding Information:
This work was financially supported by MEXT (24655147, 15H05371, 15K14937, 17K19477, 18H04538, and 19H02846 to T.K. and 16H05099 and 18H04609 to K.Hanaoka.), JST (T.K. and K.Hanaoka.), and AMED (Y.U.). T.K. was supported by The Naito Foundation and The Mochida Memorial Foundation for Medical and Pharmaceutical Research and The Tokyo Biochemical Research Foundation. PMF analysis was performed as a contract service by Apro Science. We would like to thank Gilles-Olivier Gratien for constructive comments on the manuscript.
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/9/17
Y1 - 2019/9/17
N2 - We have developed a novel method to globally monitor the enzymatic activities of biological samples based on performing the global activity analysis on a proteome separated by native electrophoresis. The study of the alteration in peptide-metabolizing enzymatic activity in colorectal tumor specimens led us to the discovery of elevated thimet oligopeptidase activity, which contributed to the faster consumption of immune-stimulating peptide neurotensin.
AB - We have developed a novel method to globally monitor the enzymatic activities of biological samples based on performing the global activity analysis on a proteome separated by native electrophoresis. The study of the alteration in peptide-metabolizing enzymatic activity in colorectal tumor specimens led us to the discovery of elevated thimet oligopeptidase activity, which contributed to the faster consumption of immune-stimulating peptide neurotensin.
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U2 - 10.1021/acs.analchem.9b03016
DO - 10.1021/acs.analchem.9b03016
M3 - Article
C2 - 31424921
AN - SCOPUS:85072299848
VL - 91
SP - 11497
EP - 11501
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 18
ER -