Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep

Frank M. Bloos, Hiroshi Morisaki, Andrea M. Neal, Claudio M. Martin, Chris C. Ellis, William J. Sibbald

Research output: Contribution to journalArticle

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Abstract

This study was undertaken to describe the metabolic O2 reserve of the coronary circulation in an awake sheep model of hyperdynamic sepsis. Forty- eight hours after sheep were randomized to either a SHAM group (n = 8) or a cecal ligation and perforation (CLP) group (n = 8), we measured hemodynamics, organ blood flows, and systemic and myocardial O2 metabolism variables at baseline and through four stages of progressive hypoxia. A significant elevation in arterial lactate levels occurred at a higher O2 delivery in the CLP group (527 ± 55 ml/min/m2) than in the SHAM group (357 ± 29 ml/min/m2, p < 0.05). The heart's metabolic O2 reserve (difference in circulatory determinants of O2 availability between baseline and where O2 uptake could not be sustained) was exhausted at an O2 content of 56.9 ± 4.2 ml O2/L in SHAM sheep and 79.6 ± 7.2 ml O2/L (p < 0.05) in CLP sheep. An increase in coronary blood flow was three times greater in SHAM than in CLP animals. Myocardial O2 extraction increased with hypoxia in SHAM sheep (0.78 ± 0.03 to 0.88 ± 0.02, p < 0.05), but not in CLP sheep (0.79 ± 0.02 to 0.80 ± 0.04). We conclude that the metabolic O2 reserve of the coronary circulation is depressed in this model of hyperdynamic sepsis as the ability to increase both coronary blood flows and myocardial O2 extraction was significantly limited.

Original languageEnglish
Pages (from-to)1577-1584
Number of pages8
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume153
Issue number5
Publication statusPublished - 1996
Externally publishedYes

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Coronary Circulation
Sheep
Sepsis
Ligation
Oxygen
Lactic Acid
Hemodynamics
salicylhydroxamic acid

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep. / Bloos, Frank M.; Morisaki, Hiroshi; Neal, Andrea M.; Martin, Claudio M.; Ellis, Chris C.; Sibbald, William J.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 153, No. 5, 1996, p. 1577-1584.

Research output: Contribution to journalArticle

Bloos, Frank M. ; Morisaki, Hiroshi ; Neal, Andrea M. ; Martin, Claudio M. ; Ellis, Chris C. ; Sibbald, William J. / Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep. In: American Journal of Respiratory and Critical Care Medicine. 1996 ; Vol. 153, No. 5. pp. 1577-1584.
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