Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep

Frank M. Bloos; Hiroshi M. Morisaki; Andrea M. Neal; Claudio M. Martin; Chris C. Ellis; William J. Sibbald

doi:10.1164/ajrccm.153.5.8630605

Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep

Frank M. Bloos, Hiroshi M. Morisaki, Andrea M. Neal, Claudio M. Martin, Chris C. Ellis, William J. Sibbald

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Abstract

This study was undertaken to describe the metabolic O₂ reserve of the coronary circulation in an awake sheep model of hyperdynamic sepsis. Forty- eight hours after sheep were randomized to either a SHAM group (n = 8) or a cecal ligation and perforation (CLP) group (n = 8), we measured hemodynamics, organ blood flows, and systemic and myocardial O₂ metabolism variables at baseline and through four stages of progressive hypoxia. A significant elevation in arterial lactate levels occurred at a higher O₂ delivery in the CLP group (527 ± 55 ml/min/m²) than in the SHAM group (357 ± 29 ml/min/m², p < 0.05). The heart's metabolic O₂ reserve (difference in circulatory determinants of O₂ availability between baseline and where O₂ uptake could not be sustained) was exhausted at an O₂ content of 56.9 ± 4.2 ml O₂/L in SHAM sheep and 79.6 ± 7.2 ml O₂/L (p < 0.05) in CLP sheep. An increase in coronary blood flow was three times greater in SHAM than in CLP animals. Myocardial O₂ extraction increased with hypoxia in SHAM sheep (0.78 ± 0.03 to 0.88 ± 0.02, p < 0.05), but not in CLP sheep (0.79 ± 0.02 to 0.80 ± 0.04). We conclude that the metabolic O₂ reserve of the coronary circulation is depressed in this model of hyperdynamic sepsis as the ability to increase both coronary blood flows and myocardial O₂ extraction was significantly limited.

Original language	English
Pages (from-to)	1577-1584
Number of pages	8
Journal	American journal of respiratory and critical care medicine
Volume	153
Issue number	5
DOIs	https://doi.org/10.1164/ajrccm.153.5.8630605
Publication status	Published - 1996
Externally published	Yes

ASJC Scopus subject areas

Pulmonary and Respiratory Medicine
Critical Care and Intensive Care Medicine

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title = "Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep",

abstract = "This study was undertaken to describe the metabolic O2 reserve of the coronary circulation in an awake sheep model of hyperdynamic sepsis. Forty- eight hours after sheep were randomized to either a SHAM group (n = 8) or a cecal ligation and perforation (CLP) group (n = 8), we measured hemodynamics, organ blood flows, and systemic and myocardial O2 metabolism variables at baseline and through four stages of progressive hypoxia. A significant elevation in arterial lactate levels occurred at a higher O2 delivery in the CLP group (527 ± 55 ml/min/m2) than in the SHAM group (357 ± 29 ml/min/m2, p < 0.05). The heart's metabolic O2 reserve (difference in circulatory determinants of O2 availability between baseline and where O2 uptake could not be sustained) was exhausted at an O2 content of 56.9 ± 4.2 ml O2/L in SHAM sheep and 79.6 ± 7.2 ml O2/L (p < 0.05) in CLP sheep. An increase in coronary blood flow was three times greater in SHAM than in CLP animals. Myocardial O2 extraction increased with hypoxia in SHAM sheep (0.78 ± 0.03 to 0.88 ± 0.02, p < 0.05), but not in CLP sheep (0.79 ± 0.02 to 0.80 ± 0.04). We conclude that the metabolic O2 reserve of the coronary circulation is depressed in this model of hyperdynamic sepsis as the ability to increase both coronary blood flows and myocardial O2 extraction was significantly limited.",

author = "Bloos, {Frank M.} and Morisaki, {Hiroshi M.} and Neal, {Andrea M.} and Martin, {Claudio M.} and Ellis, {Chris C.} and Sibbald, {William J.}",

year = "1996",

doi = "10.1164/ajrccm.153.5.8630605",

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T1 - Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep

AU - Bloos, Frank M.

AU - Morisaki, Hiroshi M.

AU - Neal, Andrea M.

AU - Martin, Claudio M.

AU - Ellis, Chris C.

AU - Sibbald, William J.

PY - 1996

Y1 - 1996

N2 - This study was undertaken to describe the metabolic O2 reserve of the coronary circulation in an awake sheep model of hyperdynamic sepsis. Forty- eight hours after sheep were randomized to either a SHAM group (n = 8) or a cecal ligation and perforation (CLP) group (n = 8), we measured hemodynamics, organ blood flows, and systemic and myocardial O2 metabolism variables at baseline and through four stages of progressive hypoxia. A significant elevation in arterial lactate levels occurred at a higher O2 delivery in the CLP group (527 ± 55 ml/min/m2) than in the SHAM group (357 ± 29 ml/min/m2, p < 0.05). The heart's metabolic O2 reserve (difference in circulatory determinants of O2 availability between baseline and where O2 uptake could not be sustained) was exhausted at an O2 content of 56.9 ± 4.2 ml O2/L in SHAM sheep and 79.6 ± 7.2 ml O2/L (p < 0.05) in CLP sheep. An increase in coronary blood flow was three times greater in SHAM than in CLP animals. Myocardial O2 extraction increased with hypoxia in SHAM sheep (0.78 ± 0.03 to 0.88 ± 0.02, p < 0.05), but not in CLP sheep (0.79 ± 0.02 to 0.80 ± 0.04). We conclude that the metabolic O2 reserve of the coronary circulation is depressed in this model of hyperdynamic sepsis as the ability to increase both coronary blood flows and myocardial O2 extraction was significantly limited.

AB - This study was undertaken to describe the metabolic O2 reserve of the coronary circulation in an awake sheep model of hyperdynamic sepsis. Forty- eight hours after sheep were randomized to either a SHAM group (n = 8) or a cecal ligation and perforation (CLP) group (n = 8), we measured hemodynamics, organ blood flows, and systemic and myocardial O2 metabolism variables at baseline and through four stages of progressive hypoxia. A significant elevation in arterial lactate levels occurred at a higher O2 delivery in the CLP group (527 ± 55 ml/min/m2) than in the SHAM group (357 ± 29 ml/min/m2, p < 0.05). The heart's metabolic O2 reserve (difference in circulatory determinants of O2 availability between baseline and where O2 uptake could not be sustained) was exhausted at an O2 content of 56.9 ± 4.2 ml O2/L in SHAM sheep and 79.6 ± 7.2 ml O2/L (p < 0.05) in CLP sheep. An increase in coronary blood flow was three times greater in SHAM than in CLP animals. Myocardial O2 extraction increased with hypoxia in SHAM sheep (0.78 ± 0.03 to 0.88 ± 0.02, p < 0.05), but not in CLP sheep (0.79 ± 0.02 to 0.80 ± 0.04). We conclude that the metabolic O2 reserve of the coronary circulation is depressed in this model of hyperdynamic sepsis as the ability to increase both coronary blood flows and myocardial O2 extraction was significantly limited.

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Sepsis depresses the metabolic oxygen reserve of the coronary circulation in mature sheep

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