Sequence analysis of cDNAs encoding precursors of starfish asterosaps

Midori Matsumoto, Annabelle V. Briones, Takuya Nishigaki, Motonori Hoshi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To understand how starfish sperm activating peptides (asterosaps) are synthesized in the ovary, we cloned cDNAs encoding asterosaps and elucidated their nucleotide sequences. The mRNA encoding asterosaps was synthesized only in the oocytes, but not in the follicle cells, and the length was 3.7 kb. The cDNA clones contained multiple isoforms of asterosaps. We assume that asterosap precursors are large prepoly-peptide chains with an unusual 'rosary-type' structure made of 10 successive similar stretches of 51-55 residues. Each stretch finishes with a 'spacer' of 17-21 residues immediately followed by the sequence of one asterosap isoform. The N-terminal of this precursor has 19-21 successive glutamine-rich repeating units. Maturation of the precursor may require endopeptidases that cleave both C- and N-sites of lysine-arginine.

Original languageEnglish
Pages (from-to)130-136
Number of pages7
JournalDevelopmental Genetics
Volume25
Issue number2
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Starfish
Sequence Analysis
Protein Isoforms
Complementary DNA
Endopeptidases
Peptides
Glutamine
Lysine
Oocytes
Arginine
Spermatozoa
Ovary
Clone Cells
Messenger RNA

Keywords

  • Acrosome reaction
  • cDNA cloning
  • Invertebrate fertilization
  • Precursor
  • Sperm activating peptide
  • Starfish

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Genetics

Cite this

Sequence analysis of cDNAs encoding precursors of starfish asterosaps. / Matsumoto, Midori; Briones, Annabelle V.; Nishigaki, Takuya; Hoshi, Motonori.

In: Developmental Genetics, Vol. 25, No. 2, 1999, p. 130-136.

Research output: Contribution to journalArticle

Matsumoto, Midori ; Briones, Annabelle V. ; Nishigaki, Takuya ; Hoshi, Motonori. / Sequence analysis of cDNAs encoding precursors of starfish asterosaps. In: Developmental Genetics. 1999 ; Vol. 25, No. 2. pp. 130-136.
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