Sequence of neuron origin and neocortical laminar fate: Relation to cell cycle of origin in the developing murine cerebral wall

Takao Takahashi, T. Goto, S. Miyama, R. S. Nowakowski, V. S. Caviness

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

Neurons destined for each region of the neocortex are known to arise approximately in an 'inside-to-outside' sequence from a pseudostratified ventricular epithelium (PVE). This sequence is initiated rostrolaterally and propagates caudomedially. Moreover, independently of location in the PVE, the neuronogenetic sequence in mouse is divisible into 11 cell cycles that occur over a 6 d period. Here we use a novel 'birth hour' method that identifies small cohorts of neurons born during a single 2 hr period, i.e., 10-20% of a single cell cycle, which corresponds to ~1.5% of the 6 d neuronogenetic period. This method shows that neurons arising with the same cycle of the 11 cycle sequence in mouse have common laminar fates even if they arise from widely separated positions on the PVE (neurons of fields 1 and 40) and therefore arise at different embryonic times. Even at this high level of temporal resolution, simultaneously arising cells occupy more than one cortical layer, and there is substantial overlap in the distributions of cells arising with successive cycles. We demonstrate additionally that the laminar representation of cells arising with a given cycle is little if at all modified over the early postnatal interval of histogenetic cell death. We infer from these findings that cell cycle is a neuronogenetic counting mechanism and that this counting mechanism is integral to subsequent processes that determine cortical laminar fate.

Original languageEnglish
Pages (from-to)10357-10371
Number of pages15
JournalJournal of Neuroscience
Volume19
Issue number23
Publication statusPublished - 1999 Dec 1

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Cell Cycle
Neurons
Epithelium
Neocortex
Cell Death
Parturition

Keywords

  • Cell cycle
  • Neocortical histogenesis
  • Neuronogenesis
  • Neuronogenetic sequence
  • Pseudostratified ventricular epithelium (PVE)
  • Transverse neuronogenetic gradient

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sequence of neuron origin and neocortical laminar fate : Relation to cell cycle of origin in the developing murine cerebral wall. / Takahashi, Takao; Goto, T.; Miyama, S.; Nowakowski, R. S.; Caviness, V. S.

In: Journal of Neuroscience, Vol. 19, No. 23, 01.12.1999, p. 10357-10371.

Research output: Contribution to journalArticle

Takahashi, Takao ; Goto, T. ; Miyama, S. ; Nowakowski, R. S. ; Caviness, V. S. / Sequence of neuron origin and neocortical laminar fate : Relation to cell cycle of origin in the developing murine cerebral wall. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 23. pp. 10357-10371.
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