Sequence of the EndoA gene encoding mouse cytokeratin and its methylation state in the CpG-rich region

Yoshitaka Tamai, Yoshihiro Takemoto, Midori Matsumoto, Takashi Morita, Aizo Matsushiro, Masami Nozaki

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

A genomic clone obtained from mouse liver DNA using a mouse cytokeratin EndoA cDNA probe revealed the complete sequence of the EndoA gene. The gene is divided into nine exons and the exon-intron pattern has been conserved compared to that of other type-II cytokeratin-encoding genes. The 5' upstream, 3' downstream and first and third introns contain potential regulatory sequences, including polyoma virus enhancer motifs (PEA1 and PEA3) and AP-1 elements. The 5' regions upstream of the EndoA EndoB and Ck8 genes contain homologous sequences surrounding the TATA boxes. In addition, a CpG dinucleotide cluster region was located around the first exon. This CpG cluster region was found to be hypomethylated in endodermal PYS-2 cells, retinoic acid-treated F9 cells, and F9 embryonal carcinoma cells, but hypermethylated in BALB/C 3T3 fibroblast cells that do not express EndoA. These findings may provide a clue to understanding the molecular mechanisms of EndoA gene expression.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalGene
Volume104
Issue number2
DOIs
Publication statusPublished - 1991 Aug 15
Externally publishedYes

Keywords

  • AP-1
  • Genomic DNA
  • exon-intron
  • intermediate filament
  • polyoma enhancer
  • teratocarcinoma

ASJC Scopus subject areas

  • Genetics

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