Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells

Seiji Okada, Kazunari Nagayoshi, Hiromitsu Nakauchi, Shin Ichi Nishikawa, Yasusada Miura, Toshio Suda

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We confirmed that murine hematopoietic stem cells express the c-kit molecule but not lymphohematopoietic lineage markers. These lineage marker-negative c-kit-positive (Lin- c-kit+) cells were further divided according to the uptake of rhodamine-123 (Rh-123). Approximately 1,000 Lin- c-kit+ rhodamine-123dull cells, which contained 4.0 ± 1.3 and 12.5 ± 1.9 day 8 and day 12 spleen colonyforming units (CFU-S), respectively, rescued the 100% of lethally irradiated mice. One third of these cells formed colonies in the presence of interleukin-3 plus erythropoietin. The time course of the hematopoietic reconstitution of this primitive hematopoietic stem cell fraction was investigated by using Ly-5 congenic mice. Although myeloid cells and B lymphocytes were detected in the peripheral blood 2 to 3 weeks after transplantation, T lymphocytes were not detected until 4 weeks after transplantation. It is generally assumed that myeloid cells and B lymphocytes grow in the bone marrow and that T lymphocytes must pass through the thymus. For the first 2 to 3 weeks after transplantation, donor-type T lymphocytes were not dominant in the thymus, and most donor type cells were CD4/CD8 double-negative or double-positive (including CD4low and CD8low). Four weeks after transplantation, donor-type T lymphocytes were dominant and the ratio of CD4/CD8 cells had recovered to the normal pattern. However, significant numbers of T lymphocytes were detected in the peripheral blood at this stage. Sequential analysis of hematopoietic reconstitution from primitive stem cells demonstrates that myeloid and B-lymphoid lineages occurred earlier than that of the T-lymphoid lineages.

Original languageEnglish
Pages (from-to)1720-1725
Number of pages6
JournalBlood
Volume81
Issue number7
Publication statusPublished - 1993 Apr 1

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T-cells
Hematopoietic Stem Cell Transplantation
Stem cells
T-Lymphocytes
Transplantation
Thymus
Lymphocytes
Myeloid Cells
Hematopoietic Stem Cells
Thymus Gland
Blood
B-Lymphocytes
Congenic Mice
Myeloid Progenitor Cells
Rhodamine 123
CD4-CD8 Ratio
Rhodamines
Interleukin-3
Erythropoietin
Bone

ASJC Scopus subject areas

  • Hematology

Cite this

Okada, S., Nagayoshi, K., Nakauchi, H., Nishikawa, S. I., Miura, Y., & Suda, T. (1993). Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells. Blood, 81(7), 1720-1725.

Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells. / Okada, Seiji; Nagayoshi, Kazunari; Nakauchi, Hiromitsu; Nishikawa, Shin Ichi; Miura, Yasusada; Suda, Toshio.

In: Blood, Vol. 81, No. 7, 01.04.1993, p. 1720-1725.

Research output: Contribution to journalArticle

Okada, S, Nagayoshi, K, Nakauchi, H, Nishikawa, SI, Miura, Y & Suda, T 1993, 'Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells', Blood, vol. 81, no. 7, pp. 1720-1725.
Okada S, Nagayoshi K, Nakauchi H, Nishikawa SI, Miura Y, Suda T. Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells. Blood. 1993 Apr 1;81(7):1720-1725.
Okada, Seiji ; Nagayoshi, Kazunari ; Nakauchi, Hiromitsu ; Nishikawa, Shin Ichi ; Miura, Yasusada ; Suda, Toshio. / Sequential analysis of hematopoietic reconstitution achieved by transplantation of hematopoietic stem cells. In: Blood. 1993 ; Vol. 81, No. 7. pp. 1720-1725.
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