Sequential generation of asymmetric lipid vesicles using a pulsed-jetting method in rotational wells

Masahide Gotanda, Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Current methods of generating asymmetric lipid vesicles produce only single types of vesicles, which poses a challenge for investigation of vesicles with different lipid leaflet combinations using a single device. Here, we describe a device for sequentially generating asymmetric lipid giant vesicles (GVs) with various combinations of asymmetric lipid leaflets. Various combinations of planar asymmetric lipid bilayers are formed by sliding and contacting the water in oil (phospholipid) (W/O) droplets in the collecting and jetting wells of our device. Next, we generate asymmetric lipid vesicles using a pulsed-jetting method. We sequentially generate three types of GVs: two asymmetric GVs containing fluorescent-conjugated phospholipids (either (i) rhodamine or (ii) BODIPY) on the outer leaflet, and (iii) a symmetric GV with phosphatidylcholine (PC) on the inner and outer leaflets. The use of asymmetric GVs with various combinations of asymmetric lipid leaflets reveals that increase in membrane phosphatidylethanolamine (PE) concentration influences cinnamycin activity and promotes the phospholipid flip-flop dynamics. This system will be useful for investigating activities of proteins or peptides on GV membranes with various combinations of lipid leaflets.

Original languageEnglish
Pages (from-to)392-397
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume261
DOIs
Publication statusPublished - 2018 May 15

Keywords

  • Artificial cell membrane
  • Asymmetric lipid vesicle
  • Lipid-protein interaction
  • Transbilayer lipid motion

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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