Sequential insertion of multiple I-SceI recognition sites at designed loci of the bacillus subtilis 168 genome

Mitsuhiro Itaya

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Bacillus subtilis 168 is the only bacterium-based host serving for the cloning of giant DNA above 1.000 kbp. As rapid verification of the genome structure is crucial during the cloning process, six of 18-base sequence recognized by endonuclease I-SceI were sequentially created in the B. subtilis 168 genome. The established method and materials should be of use for other B. subtilis derivatives.

Original languageEnglish
Pages (from-to)180-182
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume76
Issue number1
DOIs
Publication statusPublished - 2012

Keywords

  • Bacillus subtilis
  • Genome structure
  • Genome vector
  • Rare cutting enzymes

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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