Sequential production of various types of asymmetric lipid vesicles using pulse jet flow

Masahide Gotanda, Kaki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper describes a method of sequential formation of asymmetric lipid vesicles, which applies pulse jet flow to an asymmetric planar lipid bilayer with multiple lipid components formed by Droplet Split-and-Contact Method. In our previous work, we generated asymmetric lipid vesicles by deforming an asymmetric planar lipid bilayer using a pulsed jet flow. However, it was difficult to create various types or large amounts of the asymmetric lipid vesicles in a single double-well device. In this study, asymmetric planar lipid bilayers with multiple lipid components were formed by contacting various types of lipid monolayers using movable wells on a revolving table. We successfully produced two types of the asymmetric lipid vesicles containing red or green fluorescent lipids on the outer leaflet with a single device in a high-throughput manner using the proposed method. The asymmetricity of the generated vesicles was evaluated by the distribution of the fluorescence intensities. The proposed device will be applicable for generating artificial cell models with multiple asymmetric lipid components.

Original languageEnglish
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages572-574
Number of pages3
ISBN (Electronic)9781538627310
DOIs
Publication statusPublished - 2017 Jul 26
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
Duration: 2017 Jun 182017 Jun 22

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan, Province of China
CityKaohsiung
Period17/6/1817/6/22

Keywords

  • Artificial Cell Membrane
  • Asymmetric lipid bilayer
  • Asymmetric lipid vesicles
  • Liposome

ASJC Scopus subject areas

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

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  • Cite this

    Gotanda, M., Kamiya, K., Osaki, T., Fujii, S., Misawa, N., Miki, N., & Takeuchi, S. (2017). Sequential production of various types of asymmetric lipid vesicles using pulse jet flow. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 572-574). [7994113] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994113