Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships

Takashi Sawai, Akihisa Kamataki, Miwa Uzuki, Kinji Ishida, Tomohito Hanasaka, Kensuke Ochi, Takahito Hashimoto, Takashi Kubo, Akinari Morikawa, Takahiro Ochi, Koujiro Tohyama

Research output: Contribution to journal › Article

3 Citations (Scopus)

Abstract

To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.

Original languageEnglish
Pages (from-to)317-320
Number of pages4
JournalJournal of Electron Microscopy
Volume62
Issue number2
DOIs
Publication statusPublished - 2013 Apr 1

Keywords

  • 3-D
  • back-scattered image
  • cell fusion
  • double-axis EM tomography
  • rheumatoid arthritis
  • serial block-face SEM

ASJC Scopus subject areas

  • Instrumentation

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    Sawai, T., Kamataki, A., Uzuki, M., Ishida, K., Hanasaka, T., Ochi, K., Hashimoto, T., Kubo, T., Morikawa, A., Ochi, T., & Tohyama, K. (2013). Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships. Journal of Electron Microscopy, 62(2), 317-320. https://doi.org/10.1093/jmicro/dfs069