TY - JOUR
T1 - Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships
AU - Sawai, Takashi
AU - Kamataki, Akihisa
AU - Uzuki, Miwa
AU - Ishida, Kinji
AU - Hanasaka, Tomohito
AU - Ochi, Kensuke
AU - Hashimoto, Takahito
AU - Kubo, Takashi
AU - Morikawa, Akinari
AU - Ochi, Takahiro
AU - Tohyama, Koujiro
N1 - Funding Information:
This work was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan [Grant-in-Aid for Strategic Medical Science Research Center (2009-2013) to K.T.; JSPS KAKENHI Grant number 24650181 to K.T.]; and by Ministry of Health and Labor of Japan [Grant-in-Aid of Scientific Research for Allergic Disease and Immunology to T.S.].
PY - 2013/4
Y1 - 2013/4
N2 - To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.
AB - To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.
KW - 3-D
KW - back-scattered image
KW - cell fusion
KW - double-axis EM tomography
KW - rheumatoid arthritis
KW - serial block-face SEM
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U2 - 10.1093/jmicro/dfs069
DO - 10.1093/jmicro/dfs069
M3 - Article
C2 - 23081991
AN - SCOPUS:84876021430
VL - 62
SP - 317
EP - 320
JO - Microscopy (Oxford, England)
JF - Microscopy (Oxford, England)
SN - 2050-5698
IS - 2
ER -