Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships

Takashi Sawai; Akihisa Kamataki; Miwa Uzuki; Kinji Ishida; Tomohito Hanasaka; Kensuke Ochi; Takahito Hashimoto; Takashi Kubo; Akinari Morikawa; Takahiro Ochi; Koujiro Tohyama

doi:10.1093/jmicro/dfs069

Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships

Takashi Sawai, Akihisa Kamataki, Miwa Uzuki, Kinji Ishida, Tomohito Hanasaka, Kensuke Ochi, Takahito Hashimoto, Takashi Kubo, Akinari Morikawa, Takahiro Ochi, Koujiro Tohyama

Department of Orthopaedics

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.

Original language	English
Pages (from-to)	317-320
Number of pages	4
Journal	Journal of Electron Microscopy
Volume	62
Issue number	2
DOIs	https://doi.org/10.1093/jmicro/dfs069
Publication status	Published - 2013 Apr

Keywords

3-D
back-scattered image
cell fusion
double-axis EM tomography
rheumatoid arthritis
serial block-face SEM

ASJC Scopus subject areas

Instrumentation

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10.1093/jmicro/dfs069

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Sawai, T., Kamataki, A., Uzuki, M., Ishida, K., Hanasaka, T., Ochi, K., Hashimoto, T., Kubo, T., Morikawa, A., Ochi, T., & Tohyama, K. (2013). Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships. Journal of Electron Microscopy, 62(2), 317-320. https://doi.org/10.1093/jmicro/dfs069

Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships. / Sawai, Takashi; Kamataki, Akihisa; Uzuki, Miwa; Ishida, Kinji; Hanasaka, Tomohito; Ochi, Kensuke; Hashimoto, Takahito; Kubo, Takashi; Morikawa, Akinari; Ochi, Takahiro; Tohyama, Koujiro.

In: Journal of Electron Microscopy, Vol. 62, No. 2, 04.2013, p. 317-320.

Research output: Contribution to journal › Article › peer-review

Sawai, T, Kamataki, A, Uzuki, M, Ishida, K, Hanasaka, T, Ochi, K, Hashimoto, T, Kubo, T, Morikawa, A, Ochi, T & Tohyama, K 2013, 'Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships', Journal of Electron Microscopy, vol. 62, no. 2, pp. 317-320. https://doi.org/10.1093/jmicro/dfs069

Sawai, Takashi ; Kamataki, Akihisa ; Uzuki, Miwa ; Ishida, Kinji ; Hanasaka, Tomohito ; Ochi, Kensuke ; Hashimoto, Takahito ; Kubo, Takashi ; Morikawa, Akinari ; Ochi, Takahiro ; Tohyama, Koujiro. / Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships. In: Journal of Electron Microscopy. 2013 ; Vol. 62, No. 2. pp. 317-320.

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title = "Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships",

abstract = "To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.",

keywords = "3-D, back-scattered image, cell fusion, double-axis EM tomography, rheumatoid arthritis, serial block-face SEM",

author = "Takashi Sawai and Akihisa Kamataki and Miwa Uzuki and Kinji Ishida and Tomohito Hanasaka and Kensuke Ochi and Takahito Hashimoto and Takashi Kubo and Akinari Morikawa and Takahiro Ochi and Koujiro Tohyama",

note = "Funding Information: This work was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan [Grant-in-Aid for Strategic Medical Science Research Center (2009-2013) to K.T.; JSPS KAKENHI Grant number 24650181 to K.T.]; and by Ministry of Health and Labor of Japan [Grant-in-Aid of Scientific Research for Allergic Disease and Immunology to T.S.].",

year = "2013",

month = apr,

doi = "10.1093/jmicro/dfs069",

language = "English",

volume = "62",

pages = "317--320",

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AU - Sawai, Takashi

AU - Kamataki, Akihisa

AU - Uzuki, Miwa

AU - Ishida, Kinji

AU - Hanasaka, Tomohito

AU - Ochi, Kensuke

AU - Hashimoto, Takahito

AU - Kubo, Takashi

AU - Morikawa, Akinari

AU - Ochi, Takahiro

AU - Tohyama, Koujiro

N1 - Funding Information: This work was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan [Grant-in-Aid for Strategic Medical Science Research Center (2009-2013) to K.T.; JSPS KAKENHI Grant number 24650181 to K.T.]; and by Ministry of Health and Labor of Japan [Grant-in-Aid of Scientific Research for Allergic Disease and Immunology to T.S.].

PY - 2013/4

Y1 - 2013/4

N2 - To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.

AB - To evaluate the advantages of combination of two advanced electron microscopic technologies such as serial block-face scanning electron microscopy and double-axis electron beam tomography, we analyzed the three-dimensional morphology of cellular relationships between dendritic and plasma cells in the synovial membrane from patients with rheumatoid arthritis, using the combined approach.

KW - 3-D

KW - back-scattered image

KW - cell fusion

KW - double-axis EM tomography

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Serial block-face scanning electron microscopy combined with double-axis electron beam tomography provides new insight into cellular relationships

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Keywords

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