TY - JOUR
T1 - Serodiagnostic potential of chemically synthesized glycosphingolipid antigens in an enzyme-linked immunosorbent assay for alveolar echinococcosis
AU - Yamano, K.
AU - Hada, N.
AU - Yamamura, T.
AU - Takeda, T.
AU - Honma, H.
AU - Sawada, Y.
N1 - Funding Information:
This work was supported by a grant-in-aid from the Japan Society for the Promotion of Science. The authors thank Drs Mara Rosenzvit (Instituto Nacional de Enfermedades Infecciosas, ANLIS ‘Dr Carlos G. Malbaran’, Argentina) and Graciela Ines Satillan (Instituto Nacional de Microbiologia, Argentina) for kindly providing several serum samples from patients with cystic echinococcosis.
PY - 2006/12
Y1 - 2006/12
N2 - In the serodiagnosis of alveolar echinococcosis, the detection of specific reactions against not only protein but also carbohydrate antigen is useful and both antigens supplement each other. Though recombinant protein antigens have recently advanced, the preparation of carbohydrate antigen still depends on extraction from crude antigens. In the latter case, it is not conventional to obtain carbohydrate antigen as a single component for examination and research. Therefore, chemically synthesized carbohydrate antigens were prepared for serodiagnosis by the enzyme-linked immunosorbent assay (ELISA). Four antigens with the structure of glycosphingolipids from Echinococcus multilocularis were examined and one antigen, Galβ1-6(Fucα1-3)Galβ1-6Galβ1- ceramide, was found to show significant serodiagnostic potential in differentiating alveolar from cystic echinococcosis.
AB - In the serodiagnosis of alveolar echinococcosis, the detection of specific reactions against not only protein but also carbohydrate antigen is useful and both antigens supplement each other. Though recombinant protein antigens have recently advanced, the preparation of carbohydrate antigen still depends on extraction from crude antigens. In the latter case, it is not conventional to obtain carbohydrate antigen as a single component for examination and research. Therefore, chemically synthesized carbohydrate antigens were prepared for serodiagnosis by the enzyme-linked immunosorbent assay (ELISA). Four antigens with the structure of glycosphingolipids from Echinococcus multilocularis were examined and one antigen, Galβ1-6(Fucα1-3)Galβ1-6Galβ1- ceramide, was found to show significant serodiagnostic potential in differentiating alveolar from cystic echinococcosis.
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U2 - 10.1017/JOH2006370
DO - 10.1017/JOH2006370
M3 - Article
C2 - 17125548
AN - SCOPUS:33846529984
SN - 0022-149X
VL - 80
SP - 387
EP - 391
JO - Journal of Helminthology
JF - Journal of Helminthology
IS - 4
ER -