Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans

Haruka Mori, Keita Ashida, Hisashi Shidara, Tatsuya Nikai, Kohji Hotta, Kotaro Oka

Research output: Contribution to journalArticle

Abstract

Animals change their behaviors in response to external stimuli, and numerous neurotransmitters are involved in these behavioral changes. In Caenorhabditis elegans, serotonin (5- HT) affects various behaviors such as inhibition of locomotion, stimulation of egg laying, and pharyngeal pumping. Previous research has shown that the neural activity of the RID interneuron increases when the worm moves forward, and the RID is necessary for sustaining forward locomotion. However, the relationship between 5-HT and neural activity of RID, and how it modulates the behavior of the worm has not been investigated. In this article, we reveal the relationship among 5-HT, RID activity, and the behavior of worms using a custom- made tracking and imaging system. We simultaneously measured the neural activity of the RID and behavior in worms with three conditions: mock animals, animals pre-exposed to 5-HT, and 5-HT receptor mod-1 mutants. As shown in previous research, the neural activity of the RID increased during the transition from backward to forward, whereas it decreased during the transition from forward to backward in mock animals. These changes in neural activity were not observed in animals pre-exposed to 5-HT and mod-1 mutants. Moreover, RID activity was correlated with the velocity of the worm in mock animals. However, this correlation was not observed in animals pre-exposed to 5-HT and mod-1 mutants. Our results demonstrate that 5-HT modulates the activity of the RID interneuron, and we infer that the RID plays a role in modulating forward locomotion by changing its activity through 5-HT.

Original languageEnglish
Article numbere0226044
JournalPloS one
Volume14
Issue number12
DOIs
Publication statusPublished - 2019 Jan 1

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interneurons
Caenorhabditis elegans
Interneurons
serotonin
Serotonin
Animals
Locomotion
animals
locomotion
mutants
RID
Serotonin Receptors
behavior change
neurotransmitters
Research
Imaging systems
Ovum
Neurotransmitter Agents
oviposition
image analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans. / Mori, Haruka; Ashida, Keita; Shidara, Hisashi; Nikai, Tatsuya; Hotta, Kohji; Oka, Kotaro.

In: PloS one, Vol. 14, No. 12, e0226044, 01.01.2019.

Research output: Contribution to journalArticle

Mori, Haruka ; Ashida, Keita ; Shidara, Hisashi ; Nikai, Tatsuya ; Hotta, Kohji ; Oka, Kotaro. / Serotonin modulates behavior-related neural activity of RID interneuron in Caenorhabditis elegans. In: PloS one. 2019 ; Vol. 14, No. 12.
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