Serum- and animal tissue-free medium for transport and growth of Helicobacter pylori

Cindy M. Dierikx, Jaime Martodihardjo, Ernst J. Kuipers, Charles M H Hensgens, Johannes G. Kusters, Hidekazu Suzuki, Nanda De Groot, Arnoud H M Van Vliet

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The important human gastric pathogen Helicobacter pylori is the subject of many studies, and as a consequence it is frequently being transported between national and international laboratories. Unfortunately, common bacterial growth and transport media contain serum- and animal tissue-derived materials, which carry the risk of spreading infectious diseases. We have therefore developed a growth and transport medium for H. pylori, designated 'Serum- and Animal Tissue-Free Medium' (SATFM), which does not contain serum- or animal tissue-derived components. SATFM supported growth of H. pylori isolates to similar levels as obtained with serum-supplemented Brucella medium, and SATFM with 0.5% agar supported transport and storage of H. pylori strains, as 4/4 reference strains and 11/11 clinical isolates survived for at least 3 days at room temperature in SATFM, with some strains (2/15) even surviving for up to 7 days. In conclusion, SATFM can be used both as transport and growth medium for H. pylori. The formulation of SATFM may allow its use in international transport of H. pylori, and may also allow certified use in immunization studies requiring growth of H. pylori and other bacterial pathogens.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalFEMS Immunology and Medical Microbiology
Volume50
Issue number2
DOIs
Publication statusPublished - 2007 Jul

Fingerprint

Helicobacter pylori
Growth
Serum
Brucella
Agar
Communicable Diseases
Immunization
Stomach
Temperature

Keywords

  • Growth medium
  • Helicobacter
  • Survival
  • Transport medium

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Dierikx, C. M., Martodihardjo, J., Kuipers, E. J., Hensgens, C. M. H., Kusters, J. G., Suzuki, H., ... Van Vliet, A. H. M. (2007). Serum- and animal tissue-free medium for transport and growth of Helicobacter pylori. FEMS Immunology and Medical Microbiology, 50(2), 239-243. https://doi.org/10.1111/j.1574-695X.2007.00211.x

Serum- and animal tissue-free medium for transport and growth of Helicobacter pylori. / Dierikx, Cindy M.; Martodihardjo, Jaime; Kuipers, Ernst J.; Hensgens, Charles M H; Kusters, Johannes G.; Suzuki, Hidekazu; De Groot, Nanda; Van Vliet, Arnoud H M.

In: FEMS Immunology and Medical Microbiology, Vol. 50, No. 2, 07.2007, p. 239-243.

Research output: Contribution to journalArticle

Dierikx, CM, Martodihardjo, J, Kuipers, EJ, Hensgens, CMH, Kusters, JG, Suzuki, H, De Groot, N & Van Vliet, AHM 2007, 'Serum- and animal tissue-free medium for transport and growth of Helicobacter pylori', FEMS Immunology and Medical Microbiology, vol. 50, no. 2, pp. 239-243. https://doi.org/10.1111/j.1574-695X.2007.00211.x
Dierikx, Cindy M. ; Martodihardjo, Jaime ; Kuipers, Ernst J. ; Hensgens, Charles M H ; Kusters, Johannes G. ; Suzuki, Hidekazu ; De Groot, Nanda ; Van Vliet, Arnoud H M. / Serum- and animal tissue-free medium for transport and growth of Helicobacter pylori. In: FEMS Immunology and Medical Microbiology. 2007 ; Vol. 50, No. 2. pp. 239-243.
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