Serum glycerophosphate levels are increased in Japanese men with type 2 diabetes

Makoto Daimon, Tomoyoshi Soga, Atsushi Hozawa, Toshihide Oizumi, Wataru Kaino, Kaoru Takase, Shigeru Karasawa, Yumi Jimbu, Kiriko Wada, Wataru Kameda, Shinji Susa, Takamasa Kayama, Kaori Saito, Masaru Tomita, Takeo Kato

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective To identify metabolites showing changes in serum levels among Japanese male with diabetes. Methods We performed metabolite profiling by coupling capillary electrophoresis with electrospray ionization time-of-flight mass spectrometry using fasting serum samples from Japanese male subjects with diabetes (n=17), impaired glucose tolerance (IGT; n=5) and normal glucose tolerance (NGT; n=14). Results Other than the expected differences in characteristics related to abnormal glucose metabolism, the percent body fat was significantly different among subjects with diabetes, IGT and NGT (27.3±6.2, 22.2±4.5 and 19.2±6.0%, respectively, p=0.0022). Therefore, percent body fat was considered as a possible confounding factor in subsequent analyses. Of 560 metabolites detected using our platform, the levels of 74 metabolites were quantified in all of the serum samples. Significant differences between diabetes and NGT were observed for 24 metabolites. The top-ranked metabolite was glycerol-3-phophate (glycerophosphate), which was significantly higher in subjects with diabetes than in those with NGT, even after Bonferroni correction for multiple testing (11.7±3.6 vs. 6.4±1.9 μM, respectively; corrected p=0.0222). Stepwise multiple regression analyses revealed that serum glycerophosphate levels were significantly correlated with 2-h plasma glucose after a 75-g oral glucose tolerance test (r=0.553, p=0.0005), independently of other characteristics, including FPG and HbA1c. Conclusion Serum glycerophosphate levels were found to be elevated in Japanese men with diabetes, and correlated with 2-h PG, independent of FPG and HbA1c. Namely, serum glycerophosphate level at fasting condition can be a marker for predicting glucose intolerance. These results warrant further studies to evaluate the relevance of glycerophosphate in the pathophysiology of diabetes.

Original languageEnglish
Pages (from-to)545-551
Number of pages7
JournalInternal Medicine
Volume51
Issue number6
DOIs
Publication statusPublished - 2012

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Glycerophosphates
Type 2 Diabetes Mellitus
Serum
Glucose Intolerance
Glucose
Adipose Tissue
Fasting
Capillary Electrophoresis
Glucose Tolerance Test
Glycerol
Mass Spectrometry
Regression Analysis

Keywords

  • Diabetes
  • Gluconeogenesis
  • Glycerophosphate
  • Metabolite profiling
  • Postprandial hyperglycemia

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Serum glycerophosphate levels are increased in Japanese men with type 2 diabetes. / Daimon, Makoto; Soga, Tomoyoshi; Hozawa, Atsushi; Oizumi, Toshihide; Kaino, Wataru; Takase, Kaoru; Karasawa, Shigeru; Jimbu, Yumi; Wada, Kiriko; Kameda, Wataru; Susa, Shinji; Kayama, Takamasa; Saito, Kaori; Tomita, Masaru; Kato, Takeo.

In: Internal Medicine, Vol. 51, No. 6, 2012, p. 545-551.

Research output: Contribution to journalArticle

Daimon, M, Soga, T, Hozawa, A, Oizumi, T, Kaino, W, Takase, K, Karasawa, S, Jimbu, Y, Wada, K, Kameda, W, Susa, S, Kayama, T, Saito, K, Tomita, M & Kato, T 2012, 'Serum glycerophosphate levels are increased in Japanese men with type 2 diabetes', Internal Medicine, vol. 51, no. 6, pp. 545-551. https://doi.org/10.2169/internalmedicine.51.6612
Daimon, Makoto ; Soga, Tomoyoshi ; Hozawa, Atsushi ; Oizumi, Toshihide ; Kaino, Wataru ; Takase, Kaoru ; Karasawa, Shigeru ; Jimbu, Yumi ; Wada, Kiriko ; Kameda, Wataru ; Susa, Shinji ; Kayama, Takamasa ; Saito, Kaori ; Tomita, Masaru ; Kato, Takeo. / Serum glycerophosphate levels are increased in Japanese men with type 2 diabetes. In: Internal Medicine. 2012 ; Vol. 51, No. 6. pp. 545-551.
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abstract = "Objective To identify metabolites showing changes in serum levels among Japanese male with diabetes. Methods We performed metabolite profiling by coupling capillary electrophoresis with electrospray ionization time-of-flight mass spectrometry using fasting serum samples from Japanese male subjects with diabetes (n=17), impaired glucose tolerance (IGT; n=5) and normal glucose tolerance (NGT; n=14). Results Other than the expected differences in characteristics related to abnormal glucose metabolism, the percent body fat was significantly different among subjects with diabetes, IGT and NGT (27.3±6.2, 22.2±4.5 and 19.2±6.0{\%}, respectively, p=0.0022). Therefore, percent body fat was considered as a possible confounding factor in subsequent analyses. Of 560 metabolites detected using our platform, the levels of 74 metabolites were quantified in all of the serum samples. Significant differences between diabetes and NGT were observed for 24 metabolites. The top-ranked metabolite was glycerol-3-phophate (glycerophosphate), which was significantly higher in subjects with diabetes than in those with NGT, even after Bonferroni correction for multiple testing (11.7±3.6 vs. 6.4±1.9 μM, respectively; corrected p=0.0222). Stepwise multiple regression analyses revealed that serum glycerophosphate levels were significantly correlated with 2-h plasma glucose after a 75-g oral glucose tolerance test (r=0.553, p=0.0005), independently of other characteristics, including FPG and HbA1c. Conclusion Serum glycerophosphate levels were found to be elevated in Japanese men with diabetes, and correlated with 2-h PG, independent of FPG and HbA1c. Namely, serum glycerophosphate level at fasting condition can be a marker for predicting glucose intolerance. These results warrant further studies to evaluate the relevance of glycerophosphate in the pathophysiology of diabetes.",
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AU - Daimon, Makoto

AU - Soga, Tomoyoshi

AU - Hozawa, Atsushi

AU - Oizumi, Toshihide

AU - Kaino, Wataru

AU - Takase, Kaoru

AU - Karasawa, Shigeru

AU - Jimbu, Yumi

AU - Wada, Kiriko

AU - Kameda, Wataru

AU - Susa, Shinji

AU - Kayama, Takamasa

AU - Saito, Kaori

AU - Tomita, Masaru

AU - Kato, Takeo

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N2 - Objective To identify metabolites showing changes in serum levels among Japanese male with diabetes. Methods We performed metabolite profiling by coupling capillary electrophoresis with electrospray ionization time-of-flight mass spectrometry using fasting serum samples from Japanese male subjects with diabetes (n=17), impaired glucose tolerance (IGT; n=5) and normal glucose tolerance (NGT; n=14). Results Other than the expected differences in characteristics related to abnormal glucose metabolism, the percent body fat was significantly different among subjects with diabetes, IGT and NGT (27.3±6.2, 22.2±4.5 and 19.2±6.0%, respectively, p=0.0022). Therefore, percent body fat was considered as a possible confounding factor in subsequent analyses. Of 560 metabolites detected using our platform, the levels of 74 metabolites were quantified in all of the serum samples. Significant differences between diabetes and NGT were observed for 24 metabolites. The top-ranked metabolite was glycerol-3-phophate (glycerophosphate), which was significantly higher in subjects with diabetes than in those with NGT, even after Bonferroni correction for multiple testing (11.7±3.6 vs. 6.4±1.9 μM, respectively; corrected p=0.0222). Stepwise multiple regression analyses revealed that serum glycerophosphate levels were significantly correlated with 2-h plasma glucose after a 75-g oral glucose tolerance test (r=0.553, p=0.0005), independently of other characteristics, including FPG and HbA1c. Conclusion Serum glycerophosphate levels were found to be elevated in Japanese men with diabetes, and correlated with 2-h PG, independent of FPG and HbA1c. Namely, serum glycerophosphate level at fasting condition can be a marker for predicting glucose intolerance. These results warrant further studies to evaluate the relevance of glycerophosphate in the pathophysiology of diabetes.

AB - Objective To identify metabolites showing changes in serum levels among Japanese male with diabetes. Methods We performed metabolite profiling by coupling capillary electrophoresis with electrospray ionization time-of-flight mass spectrometry using fasting serum samples from Japanese male subjects with diabetes (n=17), impaired glucose tolerance (IGT; n=5) and normal glucose tolerance (NGT; n=14). Results Other than the expected differences in characteristics related to abnormal glucose metabolism, the percent body fat was significantly different among subjects with diabetes, IGT and NGT (27.3±6.2, 22.2±4.5 and 19.2±6.0%, respectively, p=0.0022). Therefore, percent body fat was considered as a possible confounding factor in subsequent analyses. Of 560 metabolites detected using our platform, the levels of 74 metabolites were quantified in all of the serum samples. Significant differences between diabetes and NGT were observed for 24 metabolites. The top-ranked metabolite was glycerol-3-phophate (glycerophosphate), which was significantly higher in subjects with diabetes than in those with NGT, even after Bonferroni correction for multiple testing (11.7±3.6 vs. 6.4±1.9 μM, respectively; corrected p=0.0222). Stepwise multiple regression analyses revealed that serum glycerophosphate levels were significantly correlated with 2-h plasma glucose after a 75-g oral glucose tolerance test (r=0.553, p=0.0005), independently of other characteristics, including FPG and HbA1c. Conclusion Serum glycerophosphate levels were found to be elevated in Japanese men with diabetes, and correlated with 2-h PG, independent of FPG and HbA1c. Namely, serum glycerophosphate level at fasting condition can be a marker for predicting glucose intolerance. These results warrant further studies to evaluate the relevance of glycerophosphate in the pathophysiology of diabetes.

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KW - Gluconeogenesis

KW - Glycerophosphate

KW - Metabolite profiling

KW - Postprandial hyperglycemia

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