Serum response factor is modulated by the SUMO-1 conjugation system

Kazuhito Matsuzaki, Takeshi Minami, Masahide Tojo, Yoshiomi Honda, Yasuhiro Uchimura, Hisato Saitoh, Hideyo Yasuda, Shinji Nagahiro, Hideyuki Saya, Mitsuyoshi Nakao

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Serum stimulation leads to activation of the serum response factor (SRF)-mediated transcription of immediate-early genes such as c-fos via various signal transduction pathways. We have previously reported that promyelocytic leukemia protein (PML) is involved in the transcriptional regulation by SRF. PML is one of the well-known substrates for modification by small ubiquitin-related modifier-1 (SUMO-1) and several SUMO-1-modified proteins associate with PML. Here, we report that SRF is modified by SUMO-1 chiefly at lysine147 within the DNA-binding domain. Substitution of this target lysine for alanine did not affect the translocation of SRF to PML-nuclear bodies. The SRF mutant augmented the transcriptional activity under Rho A-stimulated condition but not under serum-starved condition, suggesting that activated SRF is suppressed by its sumoylation. These data support the transcriptional role of SUMO-1 conjugating system in cellular serum response.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number1
DOIs
Publication statusPublished - 2003 Jun 20
Externally publishedYes

Keywords

  • PML
  • SRF
  • SUMO-1
  • Serum response
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Matsuzaki, K., Minami, T., Tojo, M., Honda, Y., Uchimura, Y., Saitoh, H., Yasuda, H., Nagahiro, S., Saya, H., & Nakao, M. (2003). Serum response factor is modulated by the SUMO-1 conjugation system. Biochemical and Biophysical Research Communications, 306(1), 32-38. https://doi.org/10.1016/S0006-291X(03)00910-0