Serum response factor is modulated by the SUMO-1 conjugation system

Kazuhito Matsuzaki; Takeshi Minami; Masahide Tojo; Yoshiomi Honda; Yasuhiro Uchimura; Hisato Saitoh; Hideyo Yasuda; Shinji Nagahiro; Hideyuki Saya; Mitsuyoshi Nakao

doi:10.1016/S0006-291X(03)00910-0

Serum response factor is modulated by the SUMO-1 conjugation system

Kazuhito Matsuzaki, Takeshi Minami, Masahide Tojo, Yoshiomi Honda, Yasuhiro Uchimura, Hisato Saitoh, Hideyo Yasuda, Shinji Nagahiro, Hideyuki Saya, Mitsuyoshi Nakao

Research output: Contribution to journal › Article › peer-review

45 Citations (Scopus)

Abstract

Serum stimulation leads to activation of the serum response factor (SRF)-mediated transcription of immediate-early genes such as c-fos via various signal transduction pathways. We have previously reported that promyelocytic leukemia protein (PML) is involved in the transcriptional regulation by SRF. PML is one of the well-known substrates for modification by small ubiquitin-related modifier-1 (SUMO-1) and several SUMO-1-modified proteins associate with PML. Here, we report that SRF is modified by SUMO-1 chiefly at lysine¹⁴⁷ within the DNA-binding domain. Substitution of this target lysine for alanine did not affect the translocation of SRF to PML-nuclear bodies. The SRF mutant augmented the transcriptional activity under Rho A-stimulated condition but not under serum-starved condition, suggesting that activated SRF is suppressed by its sumoylation. These data support the transcriptional role of SUMO-1 conjugating system in cellular serum response.

Original language	English
Pages (from-to)	32-38
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	306
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(03)00910-0
Publication status	Published - 2003 Jun 20
Externally published	Yes

Keywords

PML
SRF
SUMO-1
Serum response
Transcription

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(03)00910-0

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title = "Serum response factor is modulated by the SUMO-1 conjugation system",

abstract = "Serum stimulation leads to activation of the serum response factor (SRF)-mediated transcription of immediate-early genes such as c-fos via various signal transduction pathways. We have previously reported that promyelocytic leukemia protein (PML) is involved in the transcriptional regulation by SRF. PML is one of the well-known substrates for modification by small ubiquitin-related modifier-1 (SUMO-1) and several SUMO-1-modified proteins associate with PML. Here, we report that SRF is modified by SUMO-1 chiefly at lysine147 within the DNA-binding domain. Substitution of this target lysine for alanine did not affect the translocation of SRF to PML-nuclear bodies. The SRF mutant augmented the transcriptional activity under Rho A-stimulated condition but not under serum-starved condition, suggesting that activated SRF is suppressed by its sumoylation. These data support the transcriptional role of SUMO-1 conjugating system in cellular serum response.",

keywords = "PML, SRF, SUMO-1, Serum response, Transcription",

author = "Kazuhito Matsuzaki and Takeshi Minami and Masahide Tojo and Yoshiomi Honda and Yasuhiro Uchimura and Hisato Saitoh and Hideyo Yasuda and Shinji Nagahiro and Hideyuki Saya and Mitsuyoshi Nakao",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas, by Special Coordination Funds for Promoting Science and Technology, by Grant-in-Aid for 21st Century COE Research from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government, and by The Grant of the Princess Takamatsu Cancer Research Fund (M.N.).",

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AU - Matsuzaki, Kazuhito

AU - Minami, Takeshi

AU - Tojo, Masahide

AU - Honda, Yoshiomi

AU - Uchimura, Yasuhiro

AU - Saitoh, Hisato

AU - Yasuda, Hideyo

AU - Nagahiro, Shinji

AU - Saya, Hideyuki

AU - Nakao, Mitsuyoshi

N1 - Funding Information: This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas, by Special Coordination Funds for Promoting Science and Technology, by Grant-in-Aid for 21st Century COE Research from the Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government, and by The Grant of the Princess Takamatsu Cancer Research Fund (M.N.).

PY - 2003/6/20

Y1 - 2003/6/20

N2 - Serum stimulation leads to activation of the serum response factor (SRF)-mediated transcription of immediate-early genes such as c-fos via various signal transduction pathways. We have previously reported that promyelocytic leukemia protein (PML) is involved in the transcriptional regulation by SRF. PML is one of the well-known substrates for modification by small ubiquitin-related modifier-1 (SUMO-1) and several SUMO-1-modified proteins associate with PML. Here, we report that SRF is modified by SUMO-1 chiefly at lysine147 within the DNA-binding domain. Substitution of this target lysine for alanine did not affect the translocation of SRF to PML-nuclear bodies. The SRF mutant augmented the transcriptional activity under Rho A-stimulated condition but not under serum-starved condition, suggesting that activated SRF is suppressed by its sumoylation. These data support the transcriptional role of SUMO-1 conjugating system in cellular serum response.

AB - Serum stimulation leads to activation of the serum response factor (SRF)-mediated transcription of immediate-early genes such as c-fos via various signal transduction pathways. We have previously reported that promyelocytic leukemia protein (PML) is involved in the transcriptional regulation by SRF. PML is one of the well-known substrates for modification by small ubiquitin-related modifier-1 (SUMO-1) and several SUMO-1-modified proteins associate with PML. Here, we report that SRF is modified by SUMO-1 chiefly at lysine147 within the DNA-binding domain. Substitution of this target lysine for alanine did not affect the translocation of SRF to PML-nuclear bodies. The SRF mutant augmented the transcriptional activity under Rho A-stimulated condition but not under serum-starved condition, suggesting that activated SRF is suppressed by its sumoylation. These data support the transcriptional role of SUMO-1 conjugating system in cellular serum response.

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KW - Transcription

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Serum response factor is modulated by the SUMO-1 conjugation system

Abstract

Keywords

ASJC Scopus subject areas

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