Serum response factor is modulated by the SUMO-1 conjugation system

Kazuhito Matsuzaki, Takeshi Minami, Masahide Tojo, Yoshiomi Honda, Yasuhiro Uchimura, Hisato Saitoh, Hideyo Yasuda, Shinji Nagahiro, Hideyuki Saya, Mitsuyoshi Nakao

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Serum stimulation leads to activation of the serum response factor (SRF)-mediated transcription of immediate-early genes such as c-fos via various signal transduction pathways. We have previously reported that promyelocytic leukemia protein (PML) is involved in the transcriptional regulation by SRF. PML is one of the well-known substrates for modification by small ubiquitin-related modifier-1 (SUMO-1) and several SUMO-1-modified proteins associate with PML. Here, we report that SRF is modified by SUMO-1 chiefly at lysine147 within the DNA-binding domain. Substitution of this target lysine for alanine did not affect the translocation of SRF to PML-nuclear bodies. The SRF mutant augmented the transcriptional activity under Rho A-stimulated condition but not under serum-starved condition, suggesting that activated SRF is suppressed by its sumoylation. These data support the transcriptional role of SUMO-1 conjugating system in cellular serum response.

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume306
Issue number1
DOIs
Publication statusPublished - 2003 Jun 20
Externally publishedYes

Fingerprint

Serum Response Factor
Ubiquitin
Proteins
Serum
Sumoylation
Signal transduction
Immediate-Early Genes
Transcription
Nuclear Proteins
Alanine
Lysine
Signal Transduction
Substitution reactions
Genes
Chemical activation
Promyelocytic Leukemia Protein
DNA
Substrates

Keywords

  • PML
  • Serum response
  • SRF
  • SUMO-1
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Matsuzaki, K., Minami, T., Tojo, M., Honda, Y., Uchimura, Y., Saitoh, H., ... Nakao, M. (2003). Serum response factor is modulated by the SUMO-1 conjugation system. Biochemical and Biophysical Research Communications, 306(1), 32-38. https://doi.org/10.1016/S0006-291X(03)00910-0

Serum response factor is modulated by the SUMO-1 conjugation system. / Matsuzaki, Kazuhito; Minami, Takeshi; Tojo, Masahide; Honda, Yoshiomi; Uchimura, Yasuhiro; Saitoh, Hisato; Yasuda, Hideyo; Nagahiro, Shinji; Saya, Hideyuki; Nakao, Mitsuyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 306, No. 1, 20.06.2003, p. 32-38.

Research output: Contribution to journalArticle

Matsuzaki, K, Minami, T, Tojo, M, Honda, Y, Uchimura, Y, Saitoh, H, Yasuda, H, Nagahiro, S, Saya, H & Nakao, M 2003, 'Serum response factor is modulated by the SUMO-1 conjugation system', Biochemical and Biophysical Research Communications, vol. 306, no. 1, pp. 32-38. https://doi.org/10.1016/S0006-291X(03)00910-0
Matsuzaki, Kazuhito ; Minami, Takeshi ; Tojo, Masahide ; Honda, Yoshiomi ; Uchimura, Yasuhiro ; Saitoh, Hisato ; Yasuda, Hideyo ; Nagahiro, Shinji ; Saya, Hideyuki ; Nakao, Mitsuyoshi. / Serum response factor is modulated by the SUMO-1 conjugation system. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 1. pp. 32-38.
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