Sex-Inducing Activities of the Land Planarian Bipalium nobile Extract Fractions, Obtained Using Bioassay-Guided Fractionation, in the Freshwater Planarian Dugesia ryukyuensis

Makoto Kitamura, Hiroyuki Tanaka, Yurie Horiguchi, Sayaka Manta, Ikuma Saito, Hisashi Iwaya, Hikaru Okamoto, Nanna Nagao, Yumi Yanagihara, Yu Taguchi, Rei Tezuka, Takanobu Maezawa, Kiyono Sekii, Kazuya Kobayashi

Research output: Contribution to journalArticlepeer-review

Abstract

Sexually mature planarians produce sex-inducing substances that induce postembryonic development of hermaphroditic reproductive organs in asexual freshwater planarians. Although the sex-inducing substances may be useful for elucidating the mechanism underlying this reproductive switch, the available information is limited. The potency of sex-inducing activity is conserved, at least at the order level. Recently, we showed that the sex-inducing activity in the land planarian Bipalium nobile was much higher than that in freshwater planarians. In the present study, we performed bioassay-guided fractionation of the sex-inducing substances produced by B. nobile and propose that crucial sex-inducing activity that triggers complete sexualization for asexual worms of the freshwater planarian Dugesia ryukyuensis is produced by additive and/or synergetic effects of various sex-inducing substances involved in ovarian development. The current study provided an isolation scheme for the minimum-required combination of sex-inducing substances for producing crucial sex-inducing activity.

Original languageEnglish
Pages (from-to)544-557
Number of pages14
JournalZoological Science
Volume38
Issue number6
DOIs
Publication statusPublished - 2021 Dec 1

Keywords

  • Bipalium nobile
  • Dugesia ryukyuensis
  • asexual reproduction
  • planaria
  • sex-inducing substance
  • sexual reproduction
  • sexualization

ASJC Scopus subject areas

  • Animal Science and Zoology

Fingerprint

Dive into the research topics of 'Sex-Inducing Activities of the Land Planarian Bipalium nobile Extract Fractions, Obtained Using Bioassay-Guided Fractionation, in the Freshwater Planarian Dugesia ryukyuensis'. Together they form a unique fingerprint.

Cite this