Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction

M. Fukuyama, K. Kawakami, K. Sakai, I. Itagaki, K. Kawano, M. Handa, Y. Ikeda

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.

Original languageEnglish
JournalASAIO Transactions
Volume36
Issue number3
Publication statusPublished - 1990 Jul

Fingerprint

Platelets
Platelet Aggregation
Cell Communication
Shear stress
Tumors
Blood Platelets
Agglomeration
Cells
Apyrase
Neoplasms
Monoclonal antibodies
Viscometers
Cell Count
Monoclonal Antibodies
Light
Cones
Scanning

ASJC Scopus subject areas

  • Biophysics

Cite this

Fukuyama, M., Kawakami, K., Sakai, K., Itagaki, I., Kawano, K., Handa, M., & Ikeda, Y. (1990). Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction. ASAIO Transactions, 36(3).

Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction. / Fukuyama, M.; Kawakami, K.; Sakai, K.; Itagaki, I.; Kawano, K.; Handa, M.; Ikeda, Y.

In: ASAIO Transactions, Vol. 36, No. 3, 07.1990.

Research output: Contribution to journalArticle

Fukuyama, M, Kawakami, K, Sakai, K, Itagaki, I, Kawano, K, Handa, M & Ikeda, Y 1990, 'Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction', ASAIO Transactions, vol. 36, no. 3.
Fukuyama M, Kawakami K, Sakai K, Itagaki I, Kawano K, Handa M et al. Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction. ASAIO Transactions. 1990 Jul;36(3).
Fukuyama, M. ; Kawakami, K. ; Sakai, K. ; Itagaki, I. ; Kawano, K. ; Handa, M. ; Ikeda, Y. / Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction. In: ASAIO Transactions. 1990 ; Vol. 36, No. 3.
@article{9817bbf8ccac426a9d4956d62bf73e07,
title = "Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction",
abstract = "To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.",
author = "M. Fukuyama and K. Kawakami and K. Sakai and I. Itagaki and K. Kawano and M. Handa and Y. Ikeda",
year = "1990",
month = "7",
language = "English",
volume = "36",
journal = "ASAIO Journal",
issn = "1058-2916",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction

AU - Fukuyama, M.

AU - Kawakami, K.

AU - Sakai, K.

AU - Itagaki, I.

AU - Kawano, K.

AU - Handa, M.

AU - Ikeda, Y.

PY - 1990/7

Y1 - 1990/7

N2 - To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.

AB - To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.

UR - http://www.scopus.com/inward/record.url?scp=0025454650&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025454650&partnerID=8YFLogxK

M3 - Article

VL - 36

JO - ASAIO Journal

JF - ASAIO Journal

SN - 1058-2916

IS - 3

ER -