Abstract
To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.
| Original language | English |
|---|---|
| Journal | ASAIO Transactions |
| Volume | 36 |
| Issue number | 3 |
| Publication status | Published - 1990 Jul |
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ASJC Scopus subject areas
- Biophysics
Cite this
Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction. / Fukuyama, M.; Kawakami, K.; Sakai, K.; Itagaki, I.; Kawano, K.; Handa, M.; Ikeda, Y.
In: ASAIO Transactions, Vol. 36, No. 3, 07.1990.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Shear stress induces not only platelet aggregation but also platelet-tumor cell interaction
AU - Fukuyama, M.
AU - Kawakami, K.
AU - Sakai, K.
AU - Itagaki, I.
AU - Kawano, K.
AU - Handa, M.
AU - Ikeda, Y.
PY - 1990/7
Y1 - 1990/7
N2 - To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.
AB - To investigate the interaction between platelets and tumor cells under well-defined flow conditions, the effect of tumor cells on platelet aggregation induced by shear stress was studied using a cone and plate viscometer adapted for measuring transmitted light intensity. Aggregation was markedly enhanced by HMV-1 cells in a cell number-dependent fashion under shear stress of 12 dyne/cm2. Enhancement was not observed at a high shear stress of 108 dyne/cm2. A monoclonal antibody against GPIIb/IIIa, 7E3 completely abolished enhancement of aggregation by HMV-1. Apyrase had similar inhibitory effects. Scanning electronmicroscopy showed that direct contacts of platelets with HMV-1 cells could be demonstrated when platelet-platelet interaction was inhibited by 7E3 or apyrase. These results may indicate that, at a shear stress of 12 dyne/cm2, direct contacts of platelets and HMV-1 cells may trigger enhancement of platelet aggregation.
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M3 - Article
C2 - 2252655
AN - SCOPUS:0025454650
VL - 36
JO - ASAIO Journal
JF - ASAIO Journal
SN - 1058-2916
IS - 3
ER -