Sigma-1 receptor alters the kinetics of Kv1.3 voltage gated potassium channels but not the sensitivity to receptor ligands

Maho Kinoshita, Yoshikazu Matsuoka, Takeshi Suzuki, Jennifer Mirrielees, Jay Yang

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Sigma1 receptors (Sigma1R) are intracellular chaperone proteins that bind psychotropic drugs and also clinically used drugs such as ketamine and haloperidol. Co-expression of the Sigma1R has been reported to enhance the sensitivity of several voltage-gated ion channels to Sigma1R ligands. Kv1.3 is the predominant voltage-gated potassium channel expressed in T lymphocytes with a documented role in immune activation. To gain a better understanding of Sigma1R modulation of Kv ion channels, we investigated the effects of Sigma1R co-expression on Kv1.3 physiology and pharmacology in ion channels expressed in Xenopus oocytes. We also explored the protein domains of Kv1.3 necessary for protein:protein interaction between Kv1.3 and Sigma1R through co-immunoprecipitation studies. Slowly inactivating outward-going currents consistent with Kv1.3 expression were elicited on step depolarizations. The current characterized by Erev, V1/2, and slope factor remained unchanged when co-expressed with Sigma1R. Analysis of inactivation time constant revealed a faster Kv1.3 current decay when co-expressed with Sigma1R. However the sensitivity to Sigma1R ligands remained unaltered when co-expressed with the Sigma1R in contrast to the previously reported modulation of ligand sensitivity in closely related Kv1.4 and Kv1.5 voltage gated potassium channels. Co-immunoprecipitation assays of various Kv1.3 truncation constructs indicated that the transmembrane domain of the Kv1.3 protein was responsible for the protein:protein interaction with the Sigma1R. Sigma1R likely interacts with different domains of Kv ion channel family proteins resulting in distinct modulation of different channels.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBrain Research
Volume1452
DOIs
Publication statusPublished - 2012 May 3
Externally publishedYes

Fingerprint

Kv1.3 Potassium Channel
Voltage-Gated Potassium Channels
Ligands
Ion Channels
Proteins
Immunoprecipitation
Kv1.5 Potassium Channel
Psychotropic Drugs
Ketamine
Haloperidol
Xenopus
Oocytes
sigma-1 receptor
Pharmacology
T-Lymphocytes

Keywords

  • Ion channel modulation
  • Kv1.3
  • Protein interaction
  • Sigma 1 receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Sigma-1 receptor alters the kinetics of Kv1.3 voltage gated potassium channels but not the sensitivity to receptor ligands. / Kinoshita, Maho; Matsuoka, Yoshikazu; Suzuki, Takeshi; Mirrielees, Jennifer; Yang, Jay.

In: Brain Research, Vol. 1452, 03.05.2012, p. 1-9.

Research output: Contribution to journalArticle

Kinoshita, Maho ; Matsuoka, Yoshikazu ; Suzuki, Takeshi ; Mirrielees, Jennifer ; Yang, Jay. / Sigma-1 receptor alters the kinetics of Kv1.3 voltage gated potassium channels but not the sensitivity to receptor ligands. In: Brain Research. 2012 ; Vol. 1452. pp. 1-9.
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