Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1α-induced chemotaxis in T cells

Yuichi Sekine, Osamu Ikeda, Satoshi Tsuji, Chikako Yamamoto, Ryuta Muromoto, Asuka Nanbo, Kenji Oritani, Akihiko Yoshimura, Tadashi Matsuda

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Abstract

Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains pleckstrin and Src homology 2-like domains, as well as a YXXQ motif in its C-terminal region. Our previous studies revealed that STAP-2 regulates integrin-mediated T cell adhesion. In the present study, we find that STAP-2 expression affects Jurkat T cell migration after stromal cell-derived factor-1α (SDF-1α)-treatment. Furthermore, STAP-2-deficient T cells exhibit reduced cell migration after SDF-1α-treatment. Importantly, overexpression of STAP-2 in Jurkat T cells induces activation of small guanine triphosphatases, such as Rac1 and Cdc42. Regarding the mechanism for this effect, we found that STAP-2 associates with Vav1, the guanine-nucleotide exchanging factor for Rac1, and enhances downstream Vav1/Rac1 signaling. These results reveal a novel STAP-2-mediated mechanism for the regulation of SDF-1α-induced chemotaxis of T cells via activation of Vav1/Rac1 signaling.

Original languageEnglish
Pages (from-to)7966-7974
Number of pages9
JournalJournal of Immunology
Volume183
Issue number12
DOIs
Publication statusPublished - 2009 Dec 15

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sekine, Y., Ikeda, O., Tsuji, S., Yamamoto, C., Muromoto, R., Nanbo, A., Oritani, K., Yoshimura, A., & Matsuda, T. (2009). Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1α-induced chemotaxis in T cells. Journal of Immunology, 183(12), 7966-7974. https://doi.org/10.4049/jimmunol.0902096