Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4

Lei Wang, Kouichi Tabu, Taichi Kimura, Masumi Tsuda, Hua Linghu, Mishie Tanino, Sadao Kaneko, Hiroshi Nishihara, Shinya Tanaka

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Signaling adaptor protein Crk has been shown to be involved in pathogenesis of human cancers including brain tumor where Crk was reported to be overexpressed. In this study, we addressed whether Crk is indispensable for malignant phenotype of brain tumor. In 20 surgical specimens of glioma, mRNA of both CrkI and CrkII was found to be elevated in malignant tumor. To define a precise role of Crk, we have established Crk-knockdown cell lines of glioblastoma KMG4 by siRNA, and early phase of cell adhesion to laminin was found to be suppressed. Wound healing assay revealed the decreased cell motility in Crk knockdown cells, and suppression of both anchorage-dependent and -independent growth were demonstrated in these cells. Furthermore, in vivo tumor forming potential was also markedly suppressed. These results suggest that Crk is required for early attachment to laminin, cell motility, and growth of glioblastoma cell line KMG4.

Original languageEnglish
Pages (from-to)976-981
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume362
Issue number4
DOIs
Publication statusPublished - 2007 Nov 3
Externally publishedYes

Keywords

  • Adaptor
  • Adhesion
  • Brain
  • Cancer
  • Crk
  • Glioblastoma
  • Invasion
  • Motility
  • SH2
  • Signaling

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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