Significance of DNA analysis for determination of ABO blood groups from hair and nail of decomposed human remains: A comparison with phenotyping by the absorption-elution method

Kaori Ishida; Bao Li Zhu; Shigeki Sakoda; Li Quan; Shigeki Oritani; Masaki Q. Fujita; Hitoshi Maeda

doi:10.1016/S1344-6223(00)80043-7

Significance of DNA analysis for determination of ABO blood groups from hair and nail of decomposed human remains: A comparison with phenotyping by the absorption-elution method

Kaori Ishida, Bao Li Zhu, Shigeki Sakoda, Li Quan, Shigeki Oritani, Masaki Q. Fujita, Hitoshi Maeda

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Seventy samples from 35 decomposed human remains were investigated for ABO histo-blood group phenotypes and genotypes by the absorption-elution method and PCR-RFLP, respectively. Phenotypes could be determined by the absorption-elution method in all cases except for some failures to detect A and H antigens from scalp hair. Genotyping was also usually successfully performed using nails when intact samples were available. The findings using hairs appeared to depend on the postmortem interval. In this series, an inconsistency between ABO phenotyping and genotyping was observed in two cases, suggesting postmortem antigenic alteration in hair and nail. These findings suggested the usefulness of serial examinations by phenotyping and genotyping for reliable ABO blood grouping of badly decomposed remains.

Original language	English
Pages (from-to)	212-215
Number of pages	4
Journal	Legal Medicine
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/S1344-6223(00)80043-7
Publication status	Published - 2000
Externally published	Yes

Keywords

ABO blood group system
Absorption-elution method
Decomposition
Identification
PCR-RFLP
Postmortem change

ASJC Scopus subject areas

Pathology and Forensic Medicine
Issues, ethics and legal aspects

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10.1016/S1344-6223(00)80043-7

Cite this

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title = "Significance of DNA analysis for determination of ABO blood groups from hair and nail of decomposed human remains: A comparison with phenotyping by the absorption-elution method",

abstract = "Seventy samples from 35 decomposed human remains were investigated for ABO histo-blood group phenotypes and genotypes by the absorption-elution method and PCR-RFLP, respectively. Phenotypes could be determined by the absorption-elution method in all cases except for some failures to detect A and H antigens from scalp hair. Genotyping was also usually successfully performed using nails when intact samples were available. The findings using hairs appeared to depend on the postmortem interval. In this series, an inconsistency between ABO phenotyping and genotyping was observed in two cases, suggesting postmortem antigenic alteration in hair and nail. These findings suggested the usefulness of serial examinations by phenotyping and genotyping for reliable ABO blood grouping of badly decomposed remains.",

keywords = "ABO blood group system, Absorption-elution method, Decomposition, Identification, PCR-RFLP, Postmortem change",

author = "Kaori Ishida and Zhu, {Bao Li} and Shigeki Sakoda and Li Quan and Shigeki Oritani and Fujita, {Masaki Q.} and Hitoshi Maeda",

year = "2000",

doi = "10.1016/S1344-6223(00)80043-7",

language = "English",

volume = "2",

pages = "212--215",

journal = "Legal Medicine",

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publisher = "Elsevier",

number = "4",

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TY - JOUR

T1 - Significance of DNA analysis for determination of ABO blood groups from hair and nail of decomposed human remains

T2 - A comparison with phenotyping by the absorption-elution method

AU - Ishida, Kaori

AU - Zhu, Bao Li

AU - Sakoda, Shigeki

AU - Quan, Li

AU - Oritani, Shigeki

AU - Fujita, Masaki Q.

AU - Maeda, Hitoshi

PY - 2000

Y1 - 2000

N2 - Seventy samples from 35 decomposed human remains were investigated for ABO histo-blood group phenotypes and genotypes by the absorption-elution method and PCR-RFLP, respectively. Phenotypes could be determined by the absorption-elution method in all cases except for some failures to detect A and H antigens from scalp hair. Genotyping was also usually successfully performed using nails when intact samples were available. The findings using hairs appeared to depend on the postmortem interval. In this series, an inconsistency between ABO phenotyping and genotyping was observed in two cases, suggesting postmortem antigenic alteration in hair and nail. These findings suggested the usefulness of serial examinations by phenotyping and genotyping for reliable ABO blood grouping of badly decomposed remains.

AB - Seventy samples from 35 decomposed human remains were investigated for ABO histo-blood group phenotypes and genotypes by the absorption-elution method and PCR-RFLP, respectively. Phenotypes could be determined by the absorption-elution method in all cases except for some failures to detect A and H antigens from scalp hair. Genotyping was also usually successfully performed using nails when intact samples were available. The findings using hairs appeared to depend on the postmortem interval. In this series, an inconsistency between ABO phenotyping and genotyping was observed in two cases, suggesting postmortem antigenic alteration in hair and nail. These findings suggested the usefulness of serial examinations by phenotyping and genotyping for reliable ABO blood grouping of badly decomposed remains.

KW - ABO blood group system

KW - Absorption-elution method

KW - Decomposition

KW - Identification

KW - PCR-RFLP

KW - Postmortem change

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DO - 10.1016/S1344-6223(00)80043-7

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VL - 2

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ER -

Significance of DNA analysis for determination of ABO blood groups from hair and nail of decomposed human remains: A comparison with phenotyping by the absorption-elution method

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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