Significance of DNA analysis for determination of ABO blood groups from hair and nail of decomposed human remains: A comparison with phenotyping by the absorption-elution method

Kaori Ishida, Bao Li Zhu, Shigeki Sakoda, Li Quan, Shigeki Oritani, Masaki Q Fujita, Hitoshi Maeda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Seventy samples from 35 decomposed human remains were investigated for ABO histo-blood group phenotypes and genotypes by the absorption-elution method and PCR-RFLP, respectively. Phenotypes could be determined by the absorption-elution method in all cases except for some failures to detect A and H antigens from scalp hair. Genotyping was also usually successfully performed using nails when intact samples were available. The findings using hairs appeared to depend on the postmortem interval. In this series, an inconsistency between ABO phenotyping and genotyping was observed in two cases, suggesting postmortem antigenic alteration in hair and nail. These findings suggested the usefulness of serial examinations by phenotyping and genotyping for reliable ABO blood grouping of badly decomposed remains.

Original languageEnglish
Pages (from-to)212-215
Number of pages4
JournalLegal Medicine
Volume2
Issue number4
DOIs
Publication statusPublished - 2000
Externally publishedYes

Fingerprint

Blood Group Antigens
Nails
Hair
DNA
Blood Grouping and Crossmatching
Phenotype
Scalp
Restriction Fragment Length Polymorphisms
Genotype
Polymerase Chain Reaction
Body Remains

Keywords

  • ABO blood group system
  • Absorption-elution method
  • Decomposition
  • Identification
  • PCR-RFLP
  • Postmortem change

ASJC Scopus subject areas

  • Genetics
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health
  • Public Health, Environmental and Occupational Health

Cite this

Significance of DNA analysis for determination of ABO blood groups from hair and nail of decomposed human remains : A comparison with phenotyping by the absorption-elution method. / Ishida, Kaori; Zhu, Bao Li; Sakoda, Shigeki; Quan, Li; Oritani, Shigeki; Fujita, Masaki Q; Maeda, Hitoshi.

In: Legal Medicine, Vol. 2, No. 4, 2000, p. 212-215.

Research output: Contribution to journalArticle

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