Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases

Toshiharu Furukawa, M. Watanabe, T. Kubota, S. Kase, S. Fujita, T. Yamamoto, Y. Saikawa, T. H. Kuo, H. Tanino, N. Kurihara, Y. Kawano, K. Kawamoto, A. Suto, T. Ilramoto, K. Ishibiki, M. Kitajima

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The attachment of 7 human colon cancer lines transplantable into nude mice, and primary tumors and liver metastases from 30 patients with colon cancer to 4 extracellular matrix proteins (EMPs)-Matrigel, laminin, fibronectin, and type IV collagen-was evaluated using the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-2H (MTT) assay. Cancer cells from the 4 established tumor lines which produced experimental liver metastases in vivo showed significantly greater attachment to each EMP than those from the other 3 tumor lines which did not. Although there were no significant differences between attachment to EMPs of cancer cells from 15 clinical primary tumors with liver metastases and those without, attachment to each EMP of cells derived from liver metastases was significantly greater than that of the cells from the corresponding primary tumors in 8 cases for which liver metastases and primary tumors were examined simultaneously. Attachment to EMPs, which could be determined simply and rapidly using the MTT assay, is thus considered a significant factor in experimental and clinical liver metastases of human colon cancers.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalJournal of Surgical Oncology
Volume53
Issue number1
Publication statusPublished - 1993

Fingerprint

Extracellular Matrix Proteins
Colonic Neoplasms
Neoplasm Metastasis
Liver
Neoplasms
Collagen Type IV
In Vitro Techniques
Laminin
Fibronectins
Nude Mice

Keywords

  • fresh surgical specimen
  • laminin
  • Matrigel
  • MTT assay
  • type IV collagen human colon cancer xenograft

ASJC Scopus subject areas

  • Oncology
  • Surgery

Cite this

Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases. / Furukawa, Toshiharu; Watanabe, M.; Kubota, T.; Kase, S.; Fujita, S.; Yamamoto, T.; Saikawa, Y.; Kuo, T. H.; Tanino, H.; Kurihara, N.; Kawano, Y.; Kawamoto, K.; Suto, A.; Ilramoto, T.; Ishibiki, K.; Kitajima, M.

In: Journal of Surgical Oncology, Vol. 53, No. 1, 1993, p. 10-16.

Research output: Contribution to journalArticle

Furukawa, T, Watanabe, M, Kubota, T, Kase, S, Fujita, S, Yamamoto, T, Saikawa, Y, Kuo, TH, Tanino, H, Kurihara, N, Kawano, Y, Kawamoto, K, Suto, A, Ilramoto, T, Ishibiki, K & Kitajima, M 1993, 'Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases', Journal of Surgical Oncology, vol. 53, no. 1, pp. 10-16.
Furukawa, Toshiharu ; Watanabe, M. ; Kubota, T. ; Kase, S. ; Fujita, S. ; Yamamoto, T. ; Saikawa, Y. ; Kuo, T. H. ; Tanino, H. ; Kurihara, N. ; Kawano, Y. ; Kawamoto, K. ; Suto, A. ; Ilramoto, T. ; Ishibiki, K. ; Kitajima, M. / Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases. In: Journal of Surgical Oncology. 1993 ; Vol. 53, No. 1. pp. 10-16.
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abstract = "The attachment of 7 human colon cancer lines transplantable into nude mice, and primary tumors and liver metastases from 30 patients with colon cancer to 4 extracellular matrix proteins (EMPs)-Matrigel, laminin, fibronectin, and type IV collagen-was evaluated using the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-2H (MTT) assay. Cancer cells from the 4 established tumor lines which produced experimental liver metastases in vivo showed significantly greater attachment to each EMP than those from the other 3 tumor lines which did not. Although there were no significant differences between attachment to EMPs of cancer cells from 15 clinical primary tumors with liver metastases and those without, attachment to each EMP of cells derived from liver metastases was significantly greater than that of the cells from the corresponding primary tumors in 8 cases for which liver metastases and primary tumors were examined simultaneously. Attachment to EMPs, which could be determined simply and rapidly using the MTT assay, is thus considered a significant factor in experimental and clinical liver metastases of human colon cancers.",
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