Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases

Toshiharu Furukawa, Masahiko Watanabe, Tetsuro Kubota, Suguru Kase, Shin Fujita, Takaaki Yamamoto, Yoshiro Saikawa, Tsong‐Hong ‐H Kuo, Hirokazu Tanino, Naoto Kurihara, Yukio Kawano, Kiyoshi Kawamoto, Akihiko Suto, Tatsuo Teramoto, Kyuya Ishibiki, Masaki Kitajima

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13 Citations (Scopus)

Abstract

The attachment of 7 human colon cancer lines transplantable into nude mice, and primary tumors and liver metastases from 30 patients with colon cancer to 4 extracellular matrix proteins (EMPs)—Matrigel, laminin, fibronectin, and type IV collagen—was evaluated using the 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H (MTT) assay. Cancer cells from the 4 established tumor lines which produced experimental liver metastases in vivo showed significantly greater attachment to each EMP than those from the other 3 tumor lines which did not. Although there were no significant differences between attachment to EMPs of cancer cells from 15 clinical primary tumors with liver metastases and those without, attachment to each EMP of cells derived from liver metastases was significantly greater than that of the cells from the corresponding primary tumors in 8 cases for which liver metastases and primary tumors were examined simultaneously. Attachment to EMPs, which could be determined simply and rapidly using the MTT assay, is thus considered a significant factor in experimental and clinical liver metastases of human colon cancers. © 1993 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalJournal of Surgical Oncology
Volume53
Issue number1
DOIs
Publication statusPublished - 1993 May

Keywords

  • Laminin
  • MTT assay
  • Matrigel
  • fresh surgical specimen
  • type IV collagen human colon cancer xenograft

ASJC Scopus subject areas

  • Surgery
  • Oncology

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  • Cite this

    Furukawa, T., Watanabe, M., Kubota, T., Kase, S., Fujita, S., Yamamoto, T., Saikawa, Y., Kuo, TH. H., Tanino, H., Kurihara, N., Kawano, Y., Kawamoto, K., Suto, A., Teramoto, T., Ishibiki, K., & Kitajima, M. (1993). Significance of in vitro attachment of human colon cancers to extracellular matrix proteins in experimental and clinical liver metastases. Journal of Surgical Oncology, 53(1), 10-15. https://doi.org/10.1002/jso.2930530105