TY - JOUR
T1 - Significance of lymphatic invasion on regional lymph node metastasis in early gastric cancer using LYVE-1 immunohistochemical analysis
AU - Fujimoto, Ai
AU - Ishikawa, Yukio
AU - Akishima-Fukasawa, Yuri
AU - Ito, Kinji
AU - Akasaka, Yoshikiyo
AU - Tamai, Seiichi
AU - Maehara, Tadaaki
AU - Kiguchi, Hideko
AU - Ogata, Kentaro
AU - Nishimura, Chiaki
AU - Miki, Kazumasa
AU - Ishii, Toshiharu
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/1
Y1 - 2007/1
N2 - It has been reported that lymphatic invasion is a predictor for lymph node metastasis in early gastric cancer (EGC); however, it has been impossible to differentiate between lymphatic invasion and blood vessel invasion using current staining techniques. We studied the significance of lymphatic invasion on regional lymph node metastasis in EGC by using human lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) antibody, specific to lymphatic vessels, and von Willebrand factor (vWF) antibody, specific to the blood vessels, to clearly distinguish these vascular tissues. EGC tissues were obtained from 66 node-positive and 66 node-negative subjects and were matched by age and sex. These tissues were immunostained with antibodies against LYVE-1 and vWF. Multivariate logistic regression analysis demonstrated that lymphatic invasion was a significant independent predictor for regional lymph node metastasis (odds ratio, 4.667; P = .0094), whereas blood vessel invasion was not. Thus, lymphatic invasion identified by LYVE-1 antibody could predict the existence of regional lymph node metastasis in EGC.
AB - It has been reported that lymphatic invasion is a predictor for lymph node metastasis in early gastric cancer (EGC); however, it has been impossible to differentiate between lymphatic invasion and blood vessel invasion using current staining techniques. We studied the significance of lymphatic invasion on regional lymph node metastasis in EGC by using human lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) antibody, specific to lymphatic vessels, and von Willebrand factor (vWF) antibody, specific to the blood vessels, to clearly distinguish these vascular tissues. EGC tissues were obtained from 66 node-positive and 66 node-negative subjects and were matched by age and sex. These tissues were immunostained with antibodies against LYVE-1 and vWF. Multivariate logistic regression analysis demonstrated that lymphatic invasion was a significant independent predictor for regional lymph node metastasis (odds ratio, 4.667; P = .0094), whereas blood vessel invasion was not. Thus, lymphatic invasion identified by LYVE-1 antibody could predict the existence of regional lymph node metastasis in EGC.
KW - Early gastric cancer
KW - Lymph node metastasis
KW - Lymphatic invasion
KW - Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) antibody
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U2 - 10.1309/LJQ9G0X8KP17QXP3
DO - 10.1309/LJQ9G0X8KP17QXP3
M3 - Article
C2 - 17145628
AN - SCOPUS:34249033706
SN - 0002-9173
VL - 127
SP - 82
EP - 88
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 1
ER -
