Significance of lymphatic invasion on regional lymph node metastasis in early gastric cancer using LYVE-1 immunohistochemical analysis

Ai Fujimoto, Yukio Ishikawa, Yuri Akishima-Fukasawa, Kinji Ito, Yoshikiyo Akasaka, Seiichi Tamai, Tadaaki Maehara, Hideko Kiguchi, Kentaro Ogata, Chiaki Nishimura, Kazumasa Miki, Toshiharu Ishii

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

It has been reported that lymphatic invasion is a predictor for lymph node metastasis in early gastric cancer (EGC); however, it has been impossible to differentiate between lymphatic invasion and blood vessel invasion using current staining techniques. We studied the significance of lymphatic invasion on regional lymph node metastasis in EGC by using human lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) antibody, specific to lymphatic vessels, and von Willebrand factor (vWF) antibody, specific to the blood vessels, to clearly distinguish these vascular tissues. EGC tissues were obtained from 66 node-positive and 66 node-negative subjects and were matched by age and sex. These tissues were immunostained with antibodies against LYVE-1 and vWF. Multivariate logistic regression analysis demonstrated that lymphatic invasion was a significant independent predictor for regional lymph node metastasis (odds ratio, 4.667; P = .0094), whereas blood vessel invasion was not. Thus, lymphatic invasion identified by LYVE-1 antibody could predict the existence of regional lymph node metastasis in EGC.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume127
Issue number1
DOIs
Publication statusPublished - 2007 Jan
Externally publishedYes

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CD44 Antigens
Lymphatic Vessels
Stomach Neoplasms
Lymph Nodes
Blood Vessels
Neoplasm Metastasis
Antibodies
von Willebrand Factor
Logistic Models
Odds Ratio
Regression Analysis
Staining and Labeling

Keywords

  • Early gastric cancer
  • Lymph node metastasis
  • Lymphatic invasion
  • Lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) antibody

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Significance of lymphatic invasion on regional lymph node metastasis in early gastric cancer using LYVE-1 immunohistochemical analysis. / Fujimoto, Ai; Ishikawa, Yukio; Akishima-Fukasawa, Yuri; Ito, Kinji; Akasaka, Yoshikiyo; Tamai, Seiichi; Maehara, Tadaaki; Kiguchi, Hideko; Ogata, Kentaro; Nishimura, Chiaki; Miki, Kazumasa; Ishii, Toshiharu.

In: American Journal of Clinical Pathology, Vol. 127, No. 1, 01.2007, p. 82-88.

Research output: Contribution to journalArticle

Fujimoto, A, Ishikawa, Y, Akishima-Fukasawa, Y, Ito, K, Akasaka, Y, Tamai, S, Maehara, T, Kiguchi, H, Ogata, K, Nishimura, C, Miki, K & Ishii, T 2007, 'Significance of lymphatic invasion on regional lymph node metastasis in early gastric cancer using LYVE-1 immunohistochemical analysis', American Journal of Clinical Pathology, vol. 127, no. 1, pp. 82-88. https://doi.org/10.1309/LJQ9G0X8KP17QXP3
Fujimoto, Ai ; Ishikawa, Yukio ; Akishima-Fukasawa, Yuri ; Ito, Kinji ; Akasaka, Yoshikiyo ; Tamai, Seiichi ; Maehara, Tadaaki ; Kiguchi, Hideko ; Ogata, Kentaro ; Nishimura, Chiaki ; Miki, Kazumasa ; Ishii, Toshiharu. / Significance of lymphatic invasion on regional lymph node metastasis in early gastric cancer using LYVE-1 immunohistochemical analysis. In: American Journal of Clinical Pathology. 2007 ; Vol. 127, No. 1. pp. 82-88.
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